Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/114573
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Large-scale production of lentiviral vectors using multilayer cell factories |
Author: | Rout-Pitt, N.B. McCarron, A. McIntyre, C. Parsons, D. Donnelley, M. |
Citation: | Journal of Biological Methods, 2018; 5(2):e90-1-e90-9 |
Publisher: | POL Scientific |
Issue Date: | 2018 |
ISSN: | 2326-9901 2326-9901 |
Statement of Responsibility: | Nathan Rout-Pitt, Alexandra McCarron, Chantelle McIntyre, David Parsons, Martin Donnelley |
Abstract: | Lentiviral-mediated gene therapy has been proposed for the treatment of a range of diseases, and due to its genome integration properties, it offers the potential for long-lasting benefit from a once-off treatment. Production methods for pre-clinical studies in animal models, and ultimately for human clinical trials, must be capable of producing large quantities of high-quality lentiviral vector in an efficient and cost-effective manner. We report here a medium-scale method (from 1.5 L to 6 L of vector supernatant) for lentiviral vector production in adherent cell cultures using the NUNC™ EasyFill™ Cell Factory™ from Thermo Fisher Scientific. Downstream purification uses a Mustang Q XT5 anion exchange capsule from Pall, and an ultracentrifugation step to concentrate the vector. This method is capable of producing lentiviral vector with concentrated titres of 108–109 TU/ml, with reduced manual handling compared to single monolayer flask methods. |
Keywords: | Calcium phosphate; cell factories; lentiviral vector; Mustang Q; ultracentrifugation |
Rights: | © The Authors. This work is licensed under a Creative Commons Attribution 3.0 License. |
DOI: | 10.14440/jbm.2018.236 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1098127 |
Published version: | http://dx.doi.org/10.14440/jbm.2018.236 |
Appears in Collections: | Aurora harvest 8 IPAS publications Medicine publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_114573.pdf | Published Version | 1.31 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.