Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/114177
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Type: | Journal article |
Title: | Chromosome biorientation and APC activity remain uncoupled in oocytes with reduced volume |
Author: | Lane, S. Jones, K. |
Citation: | The Journal of Cell Biology, 2017; 216(12):3949-3957 |
Publisher: | Rockefeller University Press |
Issue Date: | 2017 |
ISSN: | 0021-9525 1540-8140 |
Statement of Responsibility: | Simon I.R. Lane and Keith T. Jones |
Abstract: | The spindle assembly checkpoint (SAC) prevents chromosome missegregation by coupling anaphase onset with correct chromosome attachment and tension to microtubules. It does this by generating a diffusible signal from free kinetochores into the cytoplasm, inhibiting the anaphase-promoting complex (APC). The volume in which this signal remains effective is unknown. This raises the possibility that cell volume may be the reason the SAC is weak, and chromosome segregation error-prone, in mammalian oocytes. Here, by a process of serial bisection, we analyzed the influence of oocyte volume on the ability of the SAC to inhibit bivalent segregation in meiosis I. We were able to generate oocytes with cytoplasmic volumes reduced by 86% and observed changes in APC activity consistent with increased SAC control. However, bivalent biorientation remained uncoupled from APC activity, leading to error-prone chromosome segregation. We conclude that volume is one factor contributing to SAC weakness in oocytes. However, additional factors likely uncouple chromosome biorientation with APC activity. |
Keywords: | Oocytes Kinetochores Microtubules Animals Mice, Inbred C57BL Mice Milrinone Nocodazole Gonadotropins, Equine RNA, Complementary Microinjections Chromosome Segregation Meiosis Cell Size Gene Expression Regulation Female Spindle Apparatus Anaphase-Promoting Complex-Cyclosome |
Rights: | © 2017 Lane and Jones This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http ://www .rupress .org /terms /). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https ://creativecommons .org /licenses /by -nc -sa /4 .0 /). |
DOI: | 10.1083/jcb.201606134 |
Grant ID: | http://purl.org/au-research/grants/arc/DP120100946 |
Published version: | http://dx.doi.org/10.1083/jcb.201606134 |
Appears in Collections: | Aurora harvest 3 Molecular and Biomedical Science publications |
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hdl_114177.pdf | Published Version | 1.75 MB | Adobe PDF | View/Open |
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