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Type: Journal article
Title: miR-200/375 control epithelial plasticity-associated alternative splicing by repressing the RNA-binding protein Quaking
Author: Pillman, K.A.
Phillips, C.A.
Roslan, S.
Toubia, J.
Dredge, B.K.
Bert, A.G.
Lumb, R.
Neumann, D.P.
Li, X.
Conn, S.J.
Liu, D.
Bracken, C.P.
Lawrence, D.M.
Stylianou, N.
Schreiber, A.W.
Tilley, W.D.
Hollier, B.G.
Khew-Goodall, Y.
Selth, L.A.
Goodall, G.J.
et al.
Citation: The EMBO Journal, 2018; 37(13)
Publisher: Wiley
Issue Date: 2018
ISSN: 0261-4189
Statement of
Katherine A Pillman, Caroline A Phillips, Suraya Roslan, John Toubia, B Kate Dredge, Andrew G Bert, Rachael Lumb, Daniel P Neumann, Xiaochun Li, Simon J Conn, Dawei Liu, Cameron P Bracken, David M Lawrence, Nataly Stylianou, Andreas W Schreiber, Wayne D Tilley, Brett G Hollier, Yeesim Khew-Goodall, Luke A Selth, Gregory J Goodall, Philip A Gregory
Abstract: Members of the miR-200 family are critical gatekeepers of the epithelial state, restraining expression of pro-mesenchymal genes that drive epithelial-mesenchymal transition (EMT) and contribute to metastatic cancer progression. Here, we show that miR-200c and another epithelial-enriched miRNA, miR-375, exert widespread control of alternative splicing in cancer cells by suppressing the RNA-binding protein Quaking (QKI). During EMT, QKI-5 directly binds to and regulates hundreds of alternative splicing targets and exerts pleiotropic effects, such as increasing cell migration and invasion and restraining tumour growth, without appreciably affecting mRNA levels. QKI-5 is both necessary and sufficient to direct EMT-associated alternative splicing changes, and this splicing signature is broadly conserved across many epithelial-derived cancer types. Importantly, several actin cytoskeleton-associated genes are directly targeted by both QKI and miR-200c, revealing coordinated control of alternative splicing and mRNA abundance during EMT These findings demonstrate the existence of a miR-200/miR-375/QKI axis that impacts cancer-associated epithelial cell plasticity through widespread control of alternative splicing.
Keywords: Quaking
alternative splicing
epithelial–mesenchymal transition
Rights: © 2018 The Authors. Published under the terms of the CC BY 4.0 license
DOI: 10.15252/embj.201899016
Grant ID:
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