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https://hdl.handle.net/2440/114142
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Type: | Journal article |
Title: | Genomic characterization of coagulase-negative staphylococci including methicillin-resistant Staphylococcus sciuri causing bovine mastitis |
Author: | Khazandi, M. Al-Farha, A. Coombs, G. O'Dea, M. Pang, S. Trott, D. Aviles, R. Hemmatzadeh, F. Venter, H. Ogunniyi, A. Hoare, A. Abraham, S. Petrovski, K. |
Citation: | Veterinary Microbiology, 2018; 219:17-22 |
Publisher: | Elsevier |
Issue Date: | 2018 |
ISSN: | 0378-1135 1873-2542 |
Statement of Responsibility: | Manouchehr Khazandi, Abd Al-Bar Al-Farha, Geoffrey W. Coombs, Mark O, Dea, Stanley Pang, Darren J. Trott, Ricardo R. Aviles, Farhid Hemmatzadeh, Henrietta Venter, Abiodun D. Ogunniyi, Andrew Hoare, Sam Abraham, Kiro R. Petrovski |
Abstract: | Methicillin-resistant coagulase-negative staphylococci (MRCoNS) have recently emerged as a significant cause of bovine mastitis worldwide. Here we describe the isolation of MRCoNS from cases of bovine mastitis from a single dairy farm in Australia. Fourteen CoNS isolates were identified as MRCoNS on the basis of having an oxacillin MIC of ≥0.5 μg/mL. The isolates were speciated as S. chromogenes (n = 1) S. fleurettii (n = 1), S. haemolyticus (n = 2), S. sciuri (n = 5), S. simulans (n = 1) S. succinus (n = 2) and S. xylosus (n = 2). Five of the isolates (S. fleuretti, S. haemolyticus S. sciuri and two S. succinus) were mecA-positive. We also detected a previously described S. sciuri mecA homolog in four oxacillin-resistant S. sciuri isolates. The remainder of the putative MRCoNS did not contain any mecA-related resistance determinants in their genomes. Comparative genomic analysis of three previously published S. sciuri isolates, from humans, a squirrel and a cereal crop (rice), and a representative isolate from our study demonstrated clustering and a high degree of genetic homogeneity (>95%), suggesting S. sciuri has low host specificity. In conclusion, CoNS, in particular S. sciuri, may act as a reservoir for SCCmec elements that can easily be spread between different host species by direct cross-infection. |
Keywords: | Dairy cattle Mastitis Methicillin-resistant coagulase-negative staphylococci Staphylococcus sciuri Whole genome sequencing |
Rights: | © 2018 Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.vetmic.2018.04.004 |
Grant ID: | http://purl.org/au-research/grants/arc/LP130100736 |
Published version: | http://dx.doi.org/10.1016/j.vetmic.2018.04.004 |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest 8 |
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