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https://hdl.handle.net/2440/111115
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Type: | Journal article |
Title: | A RhoA-FRET biosensor mouse for intravital imaging in normal tissue homeostasis and disease contexts |
Author: | Nobis, M. Herrmann, D. Warren, S. Kadir, S. Leung, W. Killen, M. Magenau, A. Stevenson, D. Lucas, M. Reischmann, N. Vennin, C. Conway, J. Boulghourjian, A. Zaratzian, A. Law, A. Gallego-Ortega, D. Ormandy, C. Walters, S. Grey, S. Bailey, J. et al. |
Citation: | Cell Reports, 2017; 21(1):274-288 |
Publisher: | Cell Press |
Issue Date: | 2017 |
ISSN: | 2211-1247 2211-1247 |
Abstract: | The small GTPase RhoA is involved in a variety of fundamental processes in normal tissue. Spatiotemporal control of RhoA is thought to govern mechanosensing, growth, and motility of cells, while its deregulation is associated with disease development. Here, we describe the generation of a RhoA-fluorescence resonance energy transfer (FRET) biosensor mouse and its utility for monitoring real-time activity of RhoA in a variety of native tissues in vivo. We assess changes in RhoA activity during mechanosensing of osteocytes within the bone and during neutrophil migration. We also demonstrate spatiotemporal order of RhoA activity within crypt cells of the small intestine and during different stages of mammary gestation. Subsequently, we reveal co-option of RhoA activity in both invasive breast and pancreatic cancers, and we assess drug targeting in these disease settings, illustrating the potential for utilizing this mouse to study RhoA activity in vivo in real time. |
Keywords: | FLIM-FRET actin biosensors breast cancer cell biology development immunology intravital imaging pancreatic cancer small GTPase RhoA |
Rights: | © 2017 The Author(s). |
DOI: | 10.1016/j.celrep.2017.09.022 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1043501 http://purl.org/au-research/grants/nhmrc/1089497 http://purl.org/au-research/grants/nhmrc/1105640 http://purl.org/au-research/grants/nhmrc/1129401 http://purl.org/au-research/grants/arc/FT120100880 |
Published version: | http://dx.doi.org/10.1016/j.celrep.2017.09.022 |
Appears in Collections: | Aurora harvest 3 Medicine publications |
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