Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/99711
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Type: | Journal article |
Title: | Microstructured optical fiber-based biosensors: reversible and nanoliter-scale measurement of zinc ions |
Author: | Heng, S. McDevitt, C.A. Kostecki, R. Morey, J.R. Eijkelkamp, B.A. Ebendorff-Heidepriem, H. Monro, T.M. Abell, A.D. |
Citation: | ACS Applied Materials and Interfaces, 2016; 8(20):12727-12732 |
Publisher: | American Chemical Society |
Issue Date: | 2016 |
ISSN: | 1944-8244 1944-8252 |
Statement of Responsibility: | Sabrina Heng, Christopher A. McDevitt, Roman Kostecki, Jacqueline R. Morey, Bart A. Eijkelkamp, Heike Ebendorff-Heidepriem, Tanya M. Monro, and Andrew D. Abell |
Abstract: | Sensing platforms that allow rapid and efficient detection of metal ions would have applications in disease diagnosis and study, as well as environmental sensing. Here, we report the first microstructured optical fiber-based biosensor for the reversible and nanoliter-scale measurement of metal ions. Specifically, a photoswitchable spiropyran Zn(2+) sensor is incorporated within the microenvironment of a liposome attached to microstructured optical fibers (exposed-core and suspended-core microstructured optical fibers). Both fiber-based platforms retains high selectivity of ion binding associated with a small molecule sensor, while also allowing nanoliter volume sampling and on/off switching. We have demonstrated that multiple measurements can be made on a single sample without the need to change the sensor. The ability of the new sensing platform to sense Zn(2+) in pleural lavage and nasopharynx of mice was compared to that of established ion sensing methodologies such as inductively coupled plasma mass spectrometry (ICP-MS) and a commercially available fluorophore (Fluozin-3), where the optical-fiber-based sensor provides a significant advantage in that it allows the use of nanoliter (nL) sampling when compared to ICP-MS (mL) and FluoZin-3 (μL). This work paves the way to a generic approach for developing surface-based ion sensors using a range of sensor molecules, which can be attached to a surface without the need for its chemical modification and presents an opportunity for the development of new and highly specific ion sensors for real time sensing applications. |
Keywords: | biosensor liposome microstructured optical fiber nanoscale photoswitch zinc |
Description: | Published: May 6, 2016 |
Rights: | © 2016 American Chemical Society |
DOI: | 10.1021/acsami.6b03565 |
Grant ID: | http://purl.org/au-research/grants/arc/CE140100003 http://purl.org/au-research/grants/arc/DP150101856 |
Published version: | http://dx.doi.org/10.1021/acsami.6b03565 |
Appears in Collections: | Aurora harvest 7 IPAS publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_99711.pdf | Accepted version | 871.1 kB | Adobe PDF | View/Open |
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