Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/95184
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Type: Journal article
Title: Periodontal-ligament-derived stem cells exhibit the capacity for long-term survival, self-renewal, and regeneration of multiple tissue types in vivo
Author: Menicanin, D.
Mrozik, K.
Wada, N.
Marino, V.
Shi, S.
Bartold, P.
Gronthos, S.
Citation: Stem Cells and Development, 2014; 23(9):1001-1011
Publisher: Mary Ann Liebert
Issue Date: 2014
ISSN: 1557-8534
1547-3287
Statement of
Responsibility: 
Danijela Menicanin, Krzysztof Marek Mrozik, Naohisa Wada, Victor Marino, Songtao Shi, P. Mark Bartold, and Stan Gronthos
Abstract: Primary periodontal ligament stem cells (PDLSCs) are known to possess multidifferentiation potential and exhibit an immunophenotype similar to that described for bone-marrow-derived mesenchymal stem cells. In the present study, bromo-deoxyuridine (BrdU)-labeled ovine PDLSCs implanted into immunodeficient mice survived after 8 weeks post-transplantation and exhibited the capacity to form bone/cementum-like mineralized tissue, ligament structures similar to Sharpey's fibers with an associated vasculature. To evaluate self-renewal potential, PDLSCs were recovered from harvested primary transplants 8 weeks post-transplantation that exhibit an immunophenotype and multipotential capacity comparable to primary PDLSCs. The re-derived PDLSCs isolated from primary transplants were implanted into secondary ectopic xenogeneic transplants. Histomorphological analysis demonstrated that four out of six donor re-derived PDLSC populations displayed a capacity to survive and form fibrous ligament structures and mineralized tissues associated with vasculature in vivo, although at diminished levels in comparison to primary PDLSCs. Further, the capacity for long-term survival and the potential role of PDLSCs in dental tissue regeneration were determined using an ovine preclinical periodontal defect model. Autologous ex vivo-expanded PDLSCs that were prelabeled with BrdU were seeded onto Gelfoam(®) scaffolds and then transplanted into fenestration defects surgically created in the periodontium of the second premolars. Histological assessment at 8 weeks post-implantation revealed surviving BrdU-positive PDLSCs associated with regenerated periodontium-related tissues, including cementum and bone-like structures. This is the first report to demonstrate the self-renewal capacity of PDLSCs using serial xenogeneic transplants and provides evidence of the long-term survival and tissue contribution of autologous PDLSCs in a preclinical periodontal defect model.
Keywords: Cells, Cultured
Multipotent Stem Cells
Periodontal Ligament
Animals
Mice, Inbred NOD
Sheep
Mice
Mice, SCID
Stem Cell Transplantation
Regeneration
Cell Proliferation
Cell Survival
Time Factors
Female
Heterografts
Rights: © Mary Ann Liebert
DOI: 10.1089/scd.2013.0490
Grant ID: http://purl.org/au-research/grants/nhmrc/1043994
Published version: http://dx.doi.org/10.1089/scd.2013.0490
Appears in Collections:Aurora harvest 3
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