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https://hdl.handle.net/2440/92408
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Type: | Journal article |
Title: | X-Ray fluorescence imaging and other analyses identify selenium and GPX1 as important in female reproductive function |
Author: | Ceko, M. Hummitzsch, K. Hatzirodos, N. Bonner, W. Aitken, J. Russell, D. Lane, M. Rodgers, R. Harris, H. |
Citation: | Metallomics: integrated biometal science, 2015; 7(1):71-82 |
Publisher: | Royal Society of Chemistry |
Issue Date: | 2015 |
ISSN: | 1756-5901 1756-591X |
Statement of Responsibility: | M. J. Ceko, K. Hummitzsch, N. Hatzirodos, W. M. Bonner, J. B. Aitken, D. L. Russell, M. Lane, R. J. Rodgers and H. H. Harris |
Abstract: | Studies of selenium (Se) status indicate that Se is necessary for fertility but how precisely is not known. We aimed to show that Se was important in bovine female reproductive function. The elemental distribution in the bovine ovary (n = 45 sections) was identified by X-ray fluorescence (XRF) imaging. Se was consistently localized to the granulosa cell layer of large (>10 mm) healthy follicles. Inductively Coupled Plasma - Mass Spectrometry revealed tenfold higher Se in the bovine follicle wall compared to corpora lutea. Gene expression analysis of selenoprotein genes GPX1, GPX3, VIMP and SELM in bovine granulosa cells revealed that only GPX1 was significantly up-regulated in large healthy follicles compared to the small healthy or atretic follicles (P < 0.05). Western immunoblotting identified GPX1 protein in bovine granulosa cells of large healthy follicles, but not of small healthy follicles. To assess if GPX1 was important in human follicles, cumulus cells from women undergoing IVF/ICSI with single embryo transfer were collected. Oocytes and embryos were cultured and transferred independently in 30 patients undergoing elective single embryo transfer. Gene expression of GPX1 was significantly higher in human cumulus cells from cumulus-oocyte complexes yielding a pregnancy (P < 0.05). We present the first XRF imaging of mammalian ovaries showing that Se is consistently localized to the granulosa cells of large healthy follicles. We conclude that Se and selenoproteins are elevated in large healthy follicles and may play a critical role as an antioxidant during late follicular development. |
Keywords: | Ovarian Follicle Cells, Cultured Animals Cattle Humans Selenium Glutathione Peroxidase Oligonucleotide Array Sequence Analysis Spectrometry, X-Ray Emission Gene Expression Profiling Polymerase Chain Reaction Female Cumulus Cells Glutathione Peroxidase GPX1 |
Description: | Corrected by: Erratum: X-Ray fluorescence imaging and other analyses identify selenium and GPX1 as important in female reproductive function, in Vol. 7, Issue 1, 188. The author H. H. Harris is listed with affiliation ‘c’, however, he should be listed with affiliation ‘a’; i.e. ‘The University of Adelaide’. The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers. |
Rights: | This journal is © The Royal Society of Chemistry 2015 |
DOI: | 10.1039/c4mt00228h |
Grant ID: | http://purl.org/au-research/grants/arc/DP0985807 http://purl.org/au-research/grants/arc/DP0984722 |
Published version: | http://dx.doi.org/10.1039/c4mt00228h |
Appears in Collections: | Aurora harvest 7 IPAS publications |
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