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https://hdl.handle.net/2440/77641
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Type: | Journal article |
Title: | Isolation and characterization of mesenchymal stem cell-like cells from healthy and inflamed gingival tissue: Potential use for clinical therapy |
Author: | Ge, S. Mrozik, K. Menicanin, D. Gronthos, S. Bartold, P. |
Citation: | Regenerative Medicine, 2012; 7(6):819-832 |
Publisher: | Future Medicine Ltd |
Issue Date: | 2012 |
ISSN: | 1746-0751 1746-076X |
Statement of Responsibility: | Shaohua Ge, Krzysztof Marek Mrozik, Danijela Menicanin, Stan Gronthos, & P Mark Bartold |
Abstract: | Aim: Postnatal mesenchymal stem cell (MSC)-like cells have previously been isolated and ex vivo-expanded from healthy gingival tissues. The aim of this research was to isolate and characterize MSC-like cells from inflamed gingival tissues and determine whether they retain the characteristics of MSC-like cells from healthy gingival tissues. Materials & methods: Fifteen clonal lines of MSC-like cells from three healthy gingival tissues (GMSC-H) and fifteen from three inflamed gingival tissues (GMSC-I) were generated. Bulk-cultured cell lines from healthy and inflamed gingival tissues were also established. In vitro and in vivo characterization studies of GMSC-Is were performed relative to GMSC-Hs. Results: The incidence of clonogenic colony forming units-fibroblast was comparable between healthy and inflamed gingival tissues. GMSC-H and GMSC-I clones expressed MSC-associated markers CD44, CD73, CD90, CD105 and CD166. While the population doubling capacity of GMSC-Is was reduced compared with GMSC-Hs, both populations displayed a similar capacity to undergo osteogenic, adipogenic and chondrogenic differentiation in vitro. Following subcutaneous implantation in NOD/SCID mice, both GMSC-Hs and GMSC-Is formed dense connective tissue-like structures in vivo resembling natural gingival tissue. Conclusion: MSC-like populations exist within inflamed gingival tissue that are functionally equivalent to MSC-like cells derived from healthy gingival tissue. Given the relative abundance of inflamed gingival tissue and ease of accessibility, MSC-like cells from inflamed gingival tissues represent a newly identified population of postnatal stem cells with immense potential in tissue engineering applications. |
Keywords: | differentiation gingival inflammation mesenchymal stem cells tissue engineering |
Rights: | Copyright status unknown |
DOI: | 10.2217/rme.12.61 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/627143 |
Published version: | http://dx.doi.org/10.2217/rme.12.61 |
Appears in Collections: | Aurora harvest Dentistry publications |
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