Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/73235
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dc.contributor.authorKotur, Z.en
dc.contributor.authorMacKenzie, N.en
dc.contributor.authorRamesh, S.en
dc.contributor.authorTyerman, S.en
dc.contributor.authorKaiser, B.en
dc.contributor.authorGlass, A.en
dc.date.issued2012en
dc.identifier.citationNew Phytologist, 2012; 194(3):724-731en
dc.identifier.issn0028-646Xen
dc.identifier.issn1469-8137en
dc.identifier.urihttp://hdl.handle.net/2440/73235-
dc.description.abstractInteractions between the Arabidopsis NitRate Transporter (AtNRT2.1) and Nitrate Assimilation Related protein (AtNAR2.1, also known as AtNRT3.1) have been well documented, and confirmed by the demonstration that AtNRT2.1 and AtNAR2.1 form a 150-kDa plasma membrane complex, thought to constitute the high-affinity nitrate transporter of Arabidopsis thaliana roots. Here, we have investigated interactions between the remaining AtNRT2 family members (AtNRT2.2 to AtNRT2.7) and AtNAR2.1, and their capacity for nitrate transport. Three different systems were used to examine possible interactions with AtNAR2.1: membrane yeast split-ubiquitin, bimolecular fluorescence complementation in A. thaliana protoplasts and nitrate uptake in Xenopus oocytes. All NRT2s, except for AtNRT2.7, restored growth and β-galactosidase activity in the yeast split-ubiquitin system, and split-YFP fluorescence in A. thaliana protoplasts only when co-expressed with AtNAR2.1. Thus, except for AtNRT2.7, all other NRT2 transporters interact strongly with AtNAR2.1. Co-injection into Xenopus oocytes of cRNA of all NRT2 genes together with cRNA of AtNAR2.1 resulted in statistically significant increases of ¹⁵NO₃⁻ uptake over and above that resulting from single cRNA injections.en
dc.description.statementofresponsibilityZorica Kotur, Nenah Mackenzie, Sunita Ramesh, Stephen D. Tyerman, Brent N. Kaiser and Anthony D. M. Glassen
dc.language.isoenen
dc.publisherBlackwell Publishing Ltden
dc.rights© 2012 The Authors.en
dc.subjectArabidopsis thaliana; AtNAR2.1 (AtNRT3.1); nitrate transporter; NRT2; protein interaction.en
dc.titleNitrate transport capacity of the Arabidopsis thaliana NRT2 family members and their interactions with AtNAR2.1en
dc.typeJournal articleen
dc.identifier.rmid0020118355en
dc.identifier.doi10.1111/j.1469-8137.2012.04094.xen
dc.relation.granthttp://purl.org/au-research/grants/arc/LP0776635en
dc.identifier.pubid24887-
pubs.library.collectionAgriculture, Food and Wine publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidRamesh, S. [0000-0003-2230-4737]en
dc.identifier.orcidTyerman, S. [0000-0003-2455-1643]en
Appears in Collections:Agriculture, Food and Wine publications

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