Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/6777
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Human bone-derived cells support formation of human osteoclasts from arthroplasty-derived cells in vitro
Author: Neale, S.
Fujikawa, Y.
Sabokbar, A.
Gundle, R.
Murray, D.
Graves, S.
Howie, D.
Athanasou, N.
Citation: Journal of Bone and Joint Surgery-British Volume, 2000; 82-B(6):892-900
Publisher: British Editorial Society of Bone and Joint Surgery
Issue Date: 2000
ISSN: 0301-620X
2044-5377
Statement of
Responsibility: 
S. D. Neale, Y. Fujikawa, A. Sabokbar, R. Gundle, D. W. Murray, S. E. Graves, D. W. Howie, N. A. Athanasou
Abstract: Mononuclear osteoclast precursors are present in the wear-particle-associated macrophage infiltrate found in the membrane surrounding loose implants. These cells are capable of differentiating into osteoclastic bone-resorbing cells when co-cultured with the rat osteoblast-like cell line, UMR 106, in the presence of 1,25(OH)2 vitamin D3. In order to develop an in vitro model of osteoclast differentiation which more closely parallels the cellular microenvironment at the bone-implant interface in situ, we determined whether osteoblast-like human bone-derived cells were capable of supporting the differentiation of osteoclasts from arthroplasty-derived cells and analysed the humoral conditions required for this to occur. Long-term co-culture of arthroplasty-derived cells and human trabecular-bone-derived cells (HBDCs) resulted in the formation of numerous tartrate-resistant-acid-phosphatase (TRAP) and vitronectin-receptor (VNR)-positive multinucleated cells capable of extensive resorption of lacunar bone. The addition of 1,25(OH)2 vitamin D3 was not required for the formation of osteoclasts and bone resorption. During the formation there was release of substantial levels of M-CSF and PGE2. Exogenous PGE2 (10−8 to 10−6M) was found to stimulate strongly the resorption of osteoclastic bone. Our study has shown that HBDCs are capable of supporting the formation of osteoclasts from mononuclear phagocyte precursors present in the periprosthetic tissues surrounding a loose implant. The release of M-CSF and PGE2 by activated cells at the bone-implant interface may be important for the formation of osteoclasts at sites of pathological bone resorption associated with aseptic loosening.
Keywords: Cell Line; Macrophages; Osteoclasts; Osteoblasts; Animals; Humans; Rats; Bone Resorption; Prosthesis Failure; Acid Phosphatase; Isoenzymes; Dinoprostone; Macrophage Colony-Stimulating Factor; Receptors, Vitronectin; Arthroplasty, Replacement; Coculture Techniques; Cell Differentiation; Adult; Aged; Aged, 80 and over; Middle Aged; Female; Male; Tartrate-Resistant Acid Phosphatase
Rights: © 2000 British Editorial Society of Bone and Joint Surgery
RMID: 0001000699
DOI: 10.1302/0301-620X.82B6.10175
Published version: http://www.bjj.boneandjoint.org.uk/content/82-B/6/892
Appears in Collections:Orthopaedics and Trauma publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.