Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/6728
Citations
Scopus Web of Science® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorHaynes, D.en
dc.contributor.authorHay, S.en
dc.contributor.authorRogers, S.en
dc.contributor.authorOhta, S.en
dc.contributor.authorHowie, D.en
dc.contributor.authorGraves, S.en
dc.date.issued1997en
dc.identifier.citationJournal of Bone and Joint Surgery, 1997; 79B(6):988-994en
dc.identifier.issn0301-620Xen
dc.identifier.issn2044-5377en
dc.identifier.urihttp://hdl.handle.net/2440/6728-
dc.description.abstractBone loss around replacement prostheses may be related to the activation of mononuclear phagocytes (MNP) by prosthetic wear particles. We investigated how osteoblast-like cells were regulated by human MNP stimulated by particles of prosthetic material. Particles of titanium-6-aluminium-4-vanadium (TiAIV) stimulated MNP to release interleukin (IL)-1beta, tumour necrosis factor (TNF)alpha, IL-6 and prostaglandin E2 (PGE2). All these mediators are implicated in regulating bone metabolism. Particle-activated MNP inhibited bone cell proliferation and stimulated release of IL-6 and PGE2. The number of cells expressing alkaline phosphatase, a marker associated with mature osteoblastic cells, was reduced. Experiments with blocking antibodies showed that TNFalpha was responsible for the reduction in proliferation and the numbers of cells expressing alkaline phosphatase. By contrast, IL-1beta stimulated cell proliferation and differentiation. Both IL-1beta and TNFalpha stimulated IL-6 and PGE2 release from the osteoblast-like cells. Our results suggest that, particle-activated mononuclear phagocytes can induce a change in the balance between bone formation and resorption by a number of mechanisms.en
dc.language.isoenen
dc.publisherBRITISH EDITORIAL SOC BONE JOINT SURGERYen
dc.rights© 1997 British Editorial Society of Bone and Joint Surgeryen
dc.source.urihttp://www.bjj.boneandjoint.org.uk/content/79-B/6/988en
dc.subjectBone and Bones; Cells, Cultured; Osteoblasts; Phagocytes; Humans; Bone Resorption; Titanium; Alloys; Alkaline Phosphatase; Dinoprostone; Tumor Necrosis Factor-alpha; Interleukin-1; Interleukin-6; Antibodies, Monoclonal; Culture Media, Conditioned; Prostheses and Implants; Cell Communication; Cell Division; Cell Differentiation; Gene Expression Regulation, Enzymologic; Osteogenesisen
dc.titleRegulation of bone cells by particle-activated mononuclear phagocytesen
dc.typeJournal articleen
dc.identifier.rmid0030005837en
dc.identifier.doi10.1302/0301-620X.79B6.7832en
dc.identifier.pubid69843-
pubs.library.collectionOrthopaedics and Trauma publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidHowie, D. [0000-0003-1702-3279]en
Appears in Collections:Orthopaedics and Trauma publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.