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https://hdl.handle.net/2440/60808
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Type: | Journal article |
Title: | Expression and distribution of cytokeratin 8/18 intermediate filaments in bovine antral follicles and corpus luteum: An intrinsic mechanism of resistance to apoptosis? |
Author: | Townson, D. Putnam, A. Sullivan, B. Guo, L. Irving-Rodgers, H. |
Citation: | Histology and Histopathology: cellular and molecular biology, 2010; 25(7):889-900 |
Publisher: | F Hernandez |
Issue Date: | 2010 |
ISSN: | 0213-3911 1699-5848 |
Statement of Responsibility: | David H. Townson, Amanda N. Putnam, Brian T. Sullivan, Lankai Guo, Helen F. Irving-Rodgers |
Abstract: | Apoptosis is a mechanism of cell elimination during follicular atresia and luteal regression. Recent evidence suggests sensitivity to apoptosis in some cell types is partly dependent upon cytokeratin-containing intermediate filaments. Specifically, cytokeratin 8/18 (CK8/18) filaments are thought to impart resistance to apoptosis. Here, cytokeratin filament expression within bovine ovarian follicles and corpora lutea (CL) was characterized and the potential relationship between cell-specific CK8/18 expression and apoptosis explored. Immunoprecipitation and western blot analysis confirmed CK8 associates with CK18 to form CK8/18 heterodimeric filaments within bovine ovarian cells. Immunostaining revealed populations of CK18-positive (CK18+) cells in healthy growing follicles that increased in postovulatory follicles. Atretic follicles at all stages of atresia also contained some CK18+ cells. However, no CK18+ cells were detected in primordial or primary follicles. In CL, developing CL contained a higher proportion of CK18+ cells (similar to 35%, range 30-70%) than mature CL (similar to 16%) and regressing CL (similar to 5%; P<0.05, n = 3-5 CL/stage), suggesting CK8/18 filament expression diminishes over time, as luteal cells become more susceptible to apoptosis. Dual-fluorescence labeling for CK18 and a cell death marker (TUNEL labeling) confirmed this view, demonstrating less death of CK18+ than CK18-luteal cells throughout the estrous cycle (P<0.05). The results indicate differential expression of CK8/18 filaments occurs in cells of bovine ovarian follicles and CL throughout the estrous cycle. The prevalence and cell-specific pattern of cytokeratin expression in these structures is consistent with the concept these filaments might impart resistance to apoptosis in ovarian cells as is seen in other cell types. |
Keywords: | Ovary Apoptosis Cytoskeleton |
Rights: | Copyright status unknown |
DOI: | 10.14670/hh-25.889 |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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