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https://hdl.handle.net/2440/60743
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Type: | Journal article |
Title: | Systematic characterisation of the rat and human CYP24A1 promoter |
Author: | Sharma, R. Iachini, D. Neilsen, P. Kaplan, J. Michalakas, J. Anderson, P. May, B. Morris, H. Callen, D. |
Citation: | Molecular and Cellular Endocrinology, 2010; 325(1-2):46-53 |
Publisher: | Elsevier Sci Ireland Ltd |
Issue Date: | 2010 |
ISSN: | 0303-7207 1872-8057 |
Statement of Responsibility: | R. Kumar, D.N. Iachini, P.M. Neilsen, J. Kaplan, J. Michalakas, P.H. Anderson, B.K. May, H.A. Morris and D.F. Callen |
Abstract: | The biologically active form of vitamin D, 1,25-dihydroxyvitamin D (1,25D) ligands VDR (vitamin D receptor) and binds to the vitamin D response element (VDRE) located within target genes to regulate their transcription. Previously we showed that 1,25D-mediated rat CYP24A1 induction via the two critical VDREs is dependent on a short stretch of nucleotides called vitamin D stimulating element (VSE), located approximately 30bp upstream of VDRE-1 in the rat CYP24A1 promoter. We have now undertaken systematic analysis of the human CYP24A1 and rat CYP24A1 promoters to determine if the VSE is present in the human promoter. Using electrophoretic mobility shift and dual-luciferase reporter assays, we show that the VSE is absent in the human CYP24A1 promoter. In addition, we show that 1,25D-mediated induction of human CYP24A1 is dependant upon a promoter region spanning nucleotides -470 to -392 of the human CYP24A1 promoter. |
Keywords: | Promoter CYP24A1 Vitamin D VSE Stimulation |
Rights: | Copyright © 2010 Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.mce.2010.04.023 |
Grant ID: | ARC |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/506028/description#description |
Published version: | http://dx.doi.org/10.1016/j.mce.2010.04.023 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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