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|Title:||ZccR-a MerR-like regulator from Bordetella pertussis which responds to zinc, cadmium, and cobalt|
|Citation:||Biochemical and Biophysical Research Communications, 2003; 302(4):697-702|
|Publisher:||Academic Press Inc|
|Stephen P. Kidd and Nigel L. Brown|
|Abstract:||A transcriptional regulator of the MerR family encoded by Bordetella pertussis was characterized in Escherichia coli and in vitro. Uniquely, the regulator responded specifically to Zn(II), Cd(II), and Co(II) and was named ZccR. Gel shift assays confirmed that ZccR binds to an adjacent divergent promoter possessing an elongated spacer region of 19bp between the -10 and -35 elements, and that Zn(II), Co(II), and Cd(II) reduced the protein affinity for DNA. Site-directed mutagenesis of four cysteine and six histidine residues of ZccR showed that the cysteine residues at positions 77, 112, and 122, conserved in many of the metal-responsive MerR-like regulators, were essential for induction. Mutagenesis of the histidine residues (positions 73, 87, 90, 126, 140, and 142) revealed that histidine residues at 90, 140, and 142 were required for full induction by all three metals.|
Metal responsive promoter
Bacterial gene regulator
|Rights:||Copyright © 2003 Elsevier Science (USA). All rights reserved.|
|Appears in Collections:||Aurora harvest|
Molecular and Biomedical Science publications
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