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Type: Journal article
Title: ZccR-a MerR-like regulator from Bordetella pertussis which responds to zinc, cadmium, and cobalt
Author: Kidd, S.
Brown, N.
Citation: Biochemical and Biophysical Research Communications, 2003; 302(4):697-702
Publisher: Academic Press Inc
Issue Date: 2003
ISSN: 0006-291X
Statement of
Stephen P. Kidd and Nigel L. Brown
Abstract: A transcriptional regulator of the MerR family encoded by Bordetella pertussis was characterized in Escherichia coli and in vitro. Uniquely, the regulator responded specifically to Zn(II), Cd(II), and Co(II) and was named ZccR. Gel shift assays confirmed that ZccR binds to an adjacent divergent promoter possessing an elongated spacer region of 19bp between the -10 and -35 elements, and that Zn(II), Co(II), and Cd(II) reduced the protein affinity for DNA. Site-directed mutagenesis of four cysteine and six histidine residues of ZccR showed that the cysteine residues at positions 77, 112, and 122, conserved in many of the metal-responsive MerR-like regulators, were essential for induction. Mutagenesis of the histidine residues (positions 73, 87, 90, 126, 140, and 142) revealed that histidine residues at 90, 140, and 142 were required for full induction by all three metals.
Keywords: Regulation
MerR family
Metal responsive promoter
Bacterial gene regulator
Rights: Copyright © 2003 Elsevier Science (USA). All rights reserved.
DOI: 10.1016/S0006-291X(03)00249-3
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

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