Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/5412
Type: Journal article
Title: Extratesticular sperm maturation in the brush-tail possum, Trichosurus vulpecula
Author: Cooper, N.
Holland, M.
Breed, W.
Citation: Journal of Reproduction and Fertility Supplement, 1998; 53:221-226
Publisher: JOURNALS OF REPRODUCTION FERTILITY LTD
Issue Date: 1998
ISSN: 0022-4251
Abstract: The changes in distribution of protein and sugar components in, and on, the plasmalemma of the spermatozoon during epididymal transit of a marsupial, the brush-tail possum, Trichosurus vulpecula, are described. Freeze-fracture studies indicate a change in organization of plasmalemma intramembranous particles of both the head and midpiece of the tail as the spermatozoa pass from the caput to cauda epididymides. Staining with fluorescein isothiocyanate (FITC)-lectins shows, that heads of caput spermatozoa stain with Con A, WGA, RCA120, LCA and JAC, whereas those from the cauda epididymides stain only with LCA and JAC, thus suggesting that N-acetyl-beta-D-glucosamine, alpha-D-glucose, or beta-D-galactose may either become lost or masked during epididymal transit. A reduction of alpha-D-mannose is also suggested. Collectively these results show that the plasmalemma of the spermatozoon of at least this marsupial species undergoes both protein and saccharide modification during transit of the epididymis. How these findings relate to sperm maturation in preparation for sperm-egg binding has yet to be determined.
Keywords: Epididymis; Spermatozoa; Animals; Opossums; Acetylglucosamine; Galactose; Microscopy, Electron; Electrophoresis, Polyacrylamide Gel; Freeze Fracturing; Sperm Maturation; Male
RMID: 0030006248
Appears in Collections:Anatomical Sciences publications
Environment Institute publications

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