Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/5368
Type: | Journal article |
Title: | Differential expression of an endothelial barrier antigen between the CNS and the PNS |
Author: | Lawrenson, J. Ghabriel, M. Reid, A. Gajree, T. Allt, G. |
Citation: | Journal of Anatomy, 1995; 186(1):217-221 |
Publisher: | Cambridge University Press |
Issue Date: | 1995 |
ISSN: | 0021-8782 1469-7580 |
Statement of Responsibility: | J G Lawrenson, Mounir N Ghabriel, A R Reid, T N Gajree, and G Allt |
Abstract: | A monoclonal antibody to an antigen (EBA) expressed by neural endothelial cells (EC) was used to investigate any difference in the distribution of EBA between the CNS and PNS. Pre-embedding ultrastructural cytochemistry of rat sciatic and optic nerves was undertaken using anti-EBA, detected with a silver-enhanced gold-conjugated secondary antibody. LM immunocytochemical localisation of EBA was also performed using an HRP-conjugated secondary antibody. EC of pial and parenchymal optic nerve vessels were strongly immunopositive for EBA. Vessels of the dura were negative. At the EM level EBA was observed on the EC luminal surface. In contrast, EC of sciatic nerve were either negative or only weakly immunopositive. The molecular characteristics and function of EBA are largely unknown. Therefore the functional significance of the present findings remains to be determined. |
Keywords: | blood-brain barrier blood-nerve barrier |
Rights: | Copyright © 1995 Cambridge University Press |
Published version: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1167287/ |
Appears in Collections: | Anatomical Sciences publications Aurora harvest 5 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_5368.pdf | Published version | 1.88 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.