Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/52408
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Circulating RANKL is inversely related to RANKL mRNA levels in bone in osteoarthritic males |
Author: | Findlay, D. Chehade, M. Tsangari, H. Neale, S. Hay, S. Hopwood, B. Pannach, S. O'Loughlin, P. Fazzalari, N. |
Citation: | Arthritis Research and Therapy, 2008; 10(1):1-9 |
Publisher: | BioMed Central Ltd. |
Issue Date: | 2008 |
ISSN: | 1478-6362 1478-6362 |
Statement of Responsibility: | David Findlay, Mellick Chehade, Helen Tsangari, Susan Neale, Shelley Hay, Blair Hopwood, Susan Pannach, Peter O'Loughlin and Nicola Fazzalari |
Abstract: | Introduction The relationship of circulating levels of receptor activator of nuclear factor-κB ligand (RANKL) and osteoprotegerin (OPG) with the expression of these molecules in bone has not been established. The objective of this study was to measure, in humans, the serum levels of RANKL and OPG, and the corresponding levels in bone of mRNA encoding these proteins. Methods Fasting blood samples were obtained on the day of surgery from patients presenting for hip replacement surgery for primary osteoarthritis (OA). Intraoperatively, samples of intertrochanteric trabecular bone were collected for analysis of OPG and RANKL mRNA, using real time RT-PCR. Samples were obtained from 40 patients (15 men with age range 50 to 79 years, and 25 women with age range 47 to 87 years). Serum total RANKL and free OPG levels were measured using ELISA. Results Serum OPG levels increased over the age range of this cohort. In the men RANKL mRNA levels were positively related to age, whereas serum RANKL levels were negatively related to age. Again, in the men serum RANKL levels were inversely related (r = -0.70, P = 0.007) to RANKL mRNA levels. Also in the male group, RANKL mRNA levels were associated with a number of indices of bone structure (bone volume fraction relative to bone tissue volume, specific surface of bone relative to bone tissue volume, and trabecular thickness), bone remodelling (eroded surface and osteoid surface), and biochemical markers of bone turnover (serum alkaline phosphatase and osteocalcin, and urinary deoxypyridinoline). Conclusion This is the first report to show a relationship between serum RANKL and the expression of RANKL mRNA in bone. |
Keywords: | Bone and Bones Femur Humans Osteoarthritis RNA, Messenger Sex Factors Bone Remodeling Aged Aged, 80 and over Middle Aged Female Male RANK Ligand Osteoprotegerin |
Rights: | © 2008 Findlay et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
DOI: | 10.1186/ar2348 |
Published version: | http://dx.doi.org/10.1186/ar2348 |
Appears in Collections: | Aurora harvest Orthopaedics and Trauma publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_52408.pdf | Published version | 346.91 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.