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https://hdl.handle.net/2440/51908
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dc.contributor.author | Atkins, G. | - |
dc.contributor.author | Welldon, K. | - |
dc.contributor.author | Holding, C. | - |
dc.contributor.author | Haynes, D. | - |
dc.contributor.author | Howie, D. | - |
dc.contributor.author | Findlay, D. | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Biomaterials, 2009; 30(22):3672-3681 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.issn | 1878-5905 | - |
dc.identifier.uri | http://hdl.handle.net/2440/51908 | - |
dc.description.abstract | Polyethylene (PE) wear particles are associated with the osteolysis seen in aseptic loosening that leads to orthopaedic implant failure. While cells of the monocyte/macrophage lineage are implicated, evidence is now emerging that osteoblastic cells may also be affected by PE. In this study we investigated the effect of PE particles on osteoblasts, using a novel in vitro cell culture system that was developed to juxtapose cells and PE particles, replicating the 3-dimensional (3D) environment near implants. This system allowed normal human bone-derived cells (NHBC) to undergo differentiation into a mature osteocyte-like phenotype over a 21–28-day culture period. PE particles induced an increase in mRNA expression of the osteocyte markers E11, DMP-1 and SOST/sclerostin. NHBC responded to PE particles by increasing the mRNA expression of several genes associated with osteoclast formation and activity (RANKL, IL-8 and M-CSF) and decreased the expression of the osteoclast antagonist, OPG. PE also appeared to induce a switch in the RUNX2 control of gene expression from that of promoting matrix production (type I collagen) to inducing the expression of pro-osteoclastogenic genes. These results suggest that PE particles switch mature osteoblastic cells from an anabolic to a more catabolic phenotype. This concept was further supported by the finding that PE-induced expression of RANKL mRNA in the mouse osteocyte cell line, MLO-Y4. Overall, our results suggest that PE particles directly induce a change in the phenotype of mature osteoblasts and osteocytes, consistent with the net loss of bone near orthopaedic implants. | - |
dc.description.statementofresponsibility | Gerald J. Atkins, Katie J. Welldon, Christopher A. Holding, David R. Haynes, Donald W. Howie and David M. Findlay | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Sci Ltd | - |
dc.source.uri | http://dx.doi.org/10.1016/j.biomaterials.2009.03.035 | - |
dc.subject | Polyethylene | - |
dc.subject | Osteolysis | - |
dc.subject | Human osteoblasts | - |
dc.subject | Osteocytes | - |
dc.subject | MLO-Y4 | - |
dc.subject | Wear particles | - |
dc.title | The induction of a catabolic phenotype in human primary osteoblasts and osteocytes by polyethylene particles | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.biomaterials.2009.03.035 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Atkins, G. [0000-0002-3123-9861] | - |
dc.identifier.orcid | Howie, D. [0000-0003-1702-3279] | - |
Appears in Collections: | Aurora harvest Orthopaedics and Trauma publications |
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