Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/50773
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | T cell receptor-mediated signaling induces GRP78 expression in T cells: The implications in maintaining T cell viability |
Author: | Takano, S. Ando, T. Hiramatsu, N. Kanayama, A. Maekawa, S. Ohnuma, Y. Enomoto, N. Ogawa, H. Paton, A. Paton, J. Kitamura, M. Nakao, A. |
Citation: | Biochemical and Biophysical Research Communications, 2008; 371(4):762-766 |
Publisher: | Academic Press Inc |
Issue Date: | 2008 |
ISSN: | 0006-291X 1090-2104 |
Statement of Responsibility: | Shinichi Takano, Takashi Ando, Nobuhiko Hiramatsu, Asuka Kanayama, Shinya Maekawa, Yuko Ohnuma, Nobuyuki Enomoto, Hideoki Ogawa, Adrienne W. Paton, James C. Paton, Masanori Kitamura and Atsuhito Nakao |
Abstract: | The 78-kDa glucose-regulated protein (GRP78) is an important molecular chaperone in the endoplasmic reticulum (ER) induced by various stresses. This study showed that stimulation with anti-CD3 mAb, PMA plus ionomycin, or an antigen increased the levels of GRP78 mRNA in primary T cells, which was inhibited by Ca2+ chelators EGTA and BAPTA-AM and by an inhibitor of calcineurin FK506. In addition, the specific knockdown of GRP78 protein expression induced apoptosis in mouse EL-4 T cell line associated with CHOP induction and caspase-3 activation. Furthermore, overexpression of GRP78 inhibited PMA/ionomycin-induced cell death in EL-4 cells. Collectively, GRP78 expression is induced by TCR activation via a Ca2+-dependent pathway and may play a critical role in maintaining T cell viability in the steady and TCR-activated states. These results suggest a novel regulatory mechanism and an essential function of GRP78 in T cells. |
Keywords: | GRP78/BiP T cells T cell receptor Apoptosis |
Description: | Copyright © 2008 Elsevier B.V. All rights reserved. ScienceDirect® is a registered trademark of Elsevier B.V. |
DOI: | 10.1016/j.bbrc.2008.04.132 |
Published version: | http://dx.doi.org/10.1016/j.bbrc.2008.04.132 |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.