Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/44330
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Type: Journal article
Title: P160 myb-binding protein interacts with prep1 and inhibits its transcriptional activity
Author: Diaz, V.
Mori, S.
Longobardi, E.
Menendez, G.
Ferrai, C.
Keough, R.
Bachi, A.
Blasi, F.
Citation: Molecular and Cellular Biology, 2007; 27(22):7981-7990
Publisher: Amer Soc Microbiology
Issue Date: 2007
ISSN: 0270-7306
1098-5549
Organisation: Centre for the Molecular Genetics of Development
Statement of
Responsibility: 
Víctor M. Díaz, Silvia Mori, Elena Longobardi, Guillermo Menendez, Carmelo Ferrai, Rebecca A. Keough, Angela Bachi, and Francesco Blasi
Abstract: Prep1 is known to interact in vivo with Pbx1 to regulate development and organogenesis. We have identified a novel Prep1-interacting protein, p160 c-Myb binding protein (p160). p160 and Pbx1 compete for Prep1 in vitro, and p160 inhibits Prep1-dependent HoxB2 expression in retinoic acid-treated NT2-D1 cells. The N-terminal physiologically truncated form of p160, p67, binds the sequence 63LFPLL67 in the HR1 domain of Prep1. Mutation of both L63 and L66 impairs the binding of Prep1 to both p160/p67 and Pbx1. The sequences required to bind Prep1 are mainly located in residues 51 to 151. Immunofluorescence colocalization and coimmunoprecipitation of endogenous p160 and Prep1 are induced by ActD, which translocates p160 from the nucleolus to the nucleoplasm. These data therefore show that p160 is a novel regulator of Prep1-Pbx1 transcriptional activity.
Description: Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Provenance: Published ahead of print on 17 September 2007.
DOI: 10.1128/MCB.01290-07
Appears in Collections:Aurora harvest
Centre for the Molecular Genetics of Development publications
Molecular and Biomedical Science publications

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