Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/17312
Citations
Scopus Web of Science® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorHopwood, B.en
dc.contributor.authorGronthos, S.en
dc.contributor.authorKuliwaba, J.en
dc.contributor.authorRobey, P.en
dc.contributor.authorFindlay, D.en
dc.contributor.authorFazzalari, N.en
dc.date.issued2005en
dc.identifier.citationBone, 2005; 36(4):635-644en
dc.identifier.issn8756-3282en
dc.identifier.issn1873-2763en
dc.identifier.urihttp://hdl.handle.net/2440/17312-
dc.descriptionCopyright © 2005 Elsevier Inc. All rights reserved.en
dc.description.abstractOsteoarthritis (OA) is a common age-related joint disease resulting in progressive degenerative damage to articular cartilage. The etiology of primary OA has not yet been determined. However, there is evidence supporting the hypothesis that primary OA is a disease affecting bone remodeling in addition to articular cartilage. In this study, we have used cDNA microarray analysis to compare gene expression in bone between normal (CTL) and OA individuals. Trabecular bone was sampled from the intertrochanteric region of the proximal femur, a site distal to the diseased hip joint. Total RNA was extracted from three pairs of age- and sex-matched CTL and OA bone samples, reverse-transcribed and radioactively labeled to generate cDNA probes, before hybridization with the Research Genetics GF211 human gene microarray filter. The CTL and OA samples were found to have similar levels of gene expression for more than 4000 known human genes. However, forty-one genes were identified that were differentially expressed, twofold or more, between all three CTL–OA sample pairs. Using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis, three genes, fms-like tyrosine kinase 1 (FLT1), plexin B1 (PLXNB1), and small inducible cytokine A2 (SCYA2), were confirmed to be consistently expressed at lower levels in OA, in a majority of twenty age- and sex-matched CTL–OA bone sample pairs tested. FLT1, PLXNB1, and SCYA2 have known or potential roles in angiogenesis and bone remodeling. Down-regulation of these genes is consistent with a role for bone in the pathogenesis of OA.en
dc.description.statementofresponsibilityB. Hopwood, S. Gronthos, J.S. Kuliwaba, P.G. Robey, D.M. Findlay and N.L. Fazzalarien
dc.description.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/525233/description#descriptionen
dc.language.isoenen
dc.publisherElsevier Science Incen
dc.subjectOsteoarthritis; cDNA microarray; Proximal femur; Trabecular boneen
dc.titleIdentification of differentially expressed genes between osteoarthritic and normal trabecular bone from the intertrochanteric region of the proximal femur using cDNA microarray analysisen
dc.typeJournal articleen
dc.identifier.rmid0020050399en
dc.identifier.doi10.1016/j.bone.2005.02.003en
dc.identifier.pubid55082-
pubs.library.collectionOrthopaedics and Trauma publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidGronthos, S. [0000-0002-6225-3084]en
dc.identifier.orcidKuliwaba, J. [0000-0003-1942-9299]en
Appears in Collections:Orthopaedics and Trauma publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.