Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/137692
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Type: Journal article
Title: Expression of the wheat multipathogen resistance hexose transporter Lr67res is associated with anion fluxes
Author: Milne, R.J.
Dibley, K.E.
Bose, J.
Ashton, A.R.
Ryan, P.R.
Tyerman, S.D.
Lagudah, E.S.
Citation: Plant Physiology, 2023; 192(2):1254-1267
Publisher: Oxford University Press (OUP)
Issue Date: 2023
ISSN: 0032-0889
1532-2548
Statement of
Responsibility: 
Ricky J. Milne, Katherine E. Dibley, Jayakumar Bose, Anthony R. Ashton, Peter R. Ryan, Stephen D. Tyerman, and Evans S. Lagudah
Abstract: Many disease resistance genes in wheat (Triticum aestivum L.) confer strong resistance to specific pathogen races or strains, and only a small number of genes confer multipathogen resistance. The Leaf rust resistance 67 (Lr67) gene fits into the latter category as it confers partial resistance to multiple biotrophic fungal pathogens in wheat and encodes a Sugar Transport Protein 13 (STP13) family hexose-proton symporter variant. Two mutations (G144R, V387L) in the resistant variant, Lr67res, differentiate it from the susceptible Lr67sus variant. The molecular function of the Lr67res protein is not understood, and this study aimed to broaden our knowledge on this topic. Biophysical analysis of the wheat Lr67sus and Lr67res protein variants was performed using Xenopus laevis oocytes as a heterologous expression system. Oocytes injected with Lr67sus displayed properties typically associated with proton-coupled sugar transport proteins—glucose-dependent inward currents, a Km of 110 ± 10 µM glucose, and a substrate selectivity permitting the transport of pentoses and hexoses. By contrast, Lr67res induced much larger sugar-independent inward currents in oocytes, implicating an alternative function. Since Lr67res is a mutated hexose-proton symporter, the possibility of protons underlying these currents was investigated but rejected. Instead, currents in Lr67res oocytes appeared to be dominated by anions. This conclusion was supported by electrophysiology and 36Cl− uptake studies and the similarities with oocytes expressing the known chloride channel from Torpedo marmorata, TmClC-0. This study provides insights into the function of an important disease resistance gene in wheat, which can be used to determine how this gene variant underpins disease resistance in planta.
Keywords: Oocytes
Triticum
Protons
Chlorine
Radioisotopes
Hexoses
Glucose
Monosaccharide Transport Proteins
Plant Diseases
Disease Resistance
Sugars
Description: Published June 2023
Rights: © The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits noncommercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
DOI: 10.1093/plphys/kiad104
Grant ID: http://purl.org/au-research/grants/arc/DE170100346
http://purl.org/au-research/grants/arc/CE140100008
Published version: http://dx.doi.org/10.1093/plphys/kiad104
Appears in Collections:Agriculture, Food and Wine publications

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