Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/137416
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Type: Journal article
Title: Ovule cell wall composition is a maternal determinant of grain size in barley
Author: Yang, X.
Wilkinson, L.G.
Aubert, M.K.
Houston, K.
Shirley, N.J.
Tucker, M.R.
Citation: New Phytologist, 2023; 237(6):2136-2147
Publisher: Wiley
Issue Date: 2023
ISSN: 0028-646X
1469-8137
Statement of
Responsibility: 
Xiujuan Yang, Laura G. Wilkinson, Matthew K. Aubert, Kelly Houston, Neil J. Shirley, and Matthew R. Tucker
Abstract: In cereal species, seed and grain size is influenced by growth of the ovule integuments (seed coat), the spikelet hull (lemma and palea) and the filial endosperm. It has remained unclear whether a highly conserved ovule tissue, the nucellus, has any impact on grain size. Immunolabelling revealed that the barley nucellus comprises two distinct cell types that differ in terms of cell wall homogalacturonan (HG) accumulation. Transcriptional profiling of the nucellus identified two pectin methylesterase genes, OVULE PECTIN MODIFIER 1 (OPM1) and OPM2, which are expressed in the ovule but absent from the seed. Ovules from an opm1 opm2 mutant, and plants expressing an ovule-specific pectin methylesterase inhibitor (PMEI), exhibit reduced HG accumulation. This results in changes to ovule cell size and shape, and ovules that are longer than wild-type controls. At grain maturity, this is manifested as significantly longer grain. These findings indicate that cell wall composition during ovule development acts to limit ovule and seed growth. The investigation of ovule PME and PMEI activity reveals an unexpected role of maternal tissues in controlling grain growth prior to fertilisation, one that has been lacking from models exploring improvements in grain size.
Keywords: barley
endosperm
grain
maternal
ovule
pectin
seed
Description: First published: 04 January 2023
Rights: © 2023 The Authors New Phytologist © 2023 New Phytologist Foundation. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1111/nph.18714
Grant ID: http://purl.org/au-research/grants/arc/DP180104092
http://purl.org/au-research/grants/arc/FT140100780
Published version: http://dx.doi.org/10.1111/nph.18714
Appears in Collections:Agriculture, Food and Wine publications

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