Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/136331
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Ceramide-induced integrated stress response overcomes Bcl-2 inhibitor resistance in acute myeloid leukemia. |
Author: | Lewis, A.C. Pope, V.S. Tea, M.N. Li, M. Nwosu, G.O. Nguyen, T.M. Wallington-Beddoe, C.T. Moretti, P.A. Anderson, D. Creek, D.J. Costabile, M. Ali, S.R. Thompson-Peach, C.A. Dredge, B.K. Bert, A.G. Goodall, G.J. Ekert, P.G. Brown, A.L. D'Andrea, R.J. Robinson, N. et al. |
Citation: | Blood, 2022; 139(26):3737-3751 |
Publisher: | American Society of Hematology |
Issue Date: | 2022 |
ISSN: | 0006-4971 1528-0020 |
Statement of Responsibility: | Alexander C. Lewis, Victoria S. Pope, Melinda N. Tea, Manjun Li, Gus O. Nwosu, Thao M. Nguyen, Craig T. Wallington-Beddoe, Paul A. B. Moretti, Dovile Anderson, Darren J. Creek, Maurizio Costabile, Saira R. Ali, Chloe A. L. Thompson-Peach, B. Kate Dredge, Andrew G. Bert, Gregory J. Goodall, Paul G. Ekert, Anna L. Brown, Richard D'Andrea, Nirmal Robinson, Melissa R. Pitman, Daniel Thomas, David M. Ross, Briony L. Gliddon, Jason A. Powell, and Stuart M. Pitson |
Abstract: | Inducing cell death by the sphingolipid ceramide is a potential anti-cancer strategy, but the underlying mechanisms remain poorly defined. Here, we show that triggering accumulation of ceramide in acute myeloid leukaemia (AML) cells by inhibition of sphingosine kinase induces an apoptotic integrated stress response (ISR) through protein kinase R-mediated activation of the master transcription factor ATF4. This leads to transcription of the BH3-only protein, Noxa, and degradation of the pro-survival Mcl-1 protein on which AML cells are highly dependent on for survival. Targeting this novel ISR pathway in combination with the Bcl-2 inhibitor venetoclax synergistically killed primary AML blasts, including those with venetoclax-resistant mutations, as well as immunophenotypic leukemic stem cells, and reduced leukemic engraftment in patient-derived AML xenografts. Collectively, these findings provide mechanistic insight into the anti-cancer effects of ceramide and pre-clinical evidence for new approaches to augment Bcl-2 inhibition in the therapy of AML and other cancers with high Mcl-1 dependency. |
Keywords: | Myeloid Neoplasia |
Rights: | © 2022 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved. |
DOI: | 10.1182/blood.2021013277 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1145139 http://purl.org/au-research/grants/nhmrc/GNT1184485 |
Published version: | http://dx.doi.org/10.1182/blood.2021013277 |
Appears in Collections: | Medical Sciences publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_136331.pdf | 2.7 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.