Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/134415
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Type: | Journal article |
Title: | Loss of NEDD4 causes complete XY gonadal sex reversal in mice |
Author: | Windley, S.P. Mayère, C. McGovern, A.E. Harvey, N.L. Nef, S. Schwarz, Q. Kumar, S. Wilhelm, D. |
Citation: | Cell Death and Disease, 2022; 13(1):75-1-75-10 |
Publisher: | Springer Nature |
Issue Date: | 2022 |
ISSN: | 2041-4889 2041-4889 |
Statement of Responsibility: | Simon P. Windley, Chloé Mayère, Alice E. McGovern, Natasha L. Harvey, Serge Nef, Quenten Schwarz, Sharad Kumar and Dagmar Wilhelm |
Abstract: | Gonadogenesis is the process wherein two morphologically distinct organs, the testis and the ovary, arise from a common precursor. In mammals, maleness is driven by the expression of Sry. SRY subsequently upregulates the related family member Sox9 which is responsible for initiating testis differentiation while repressing factors critical to ovarian development such as FOXL2 and β-catenin. Here, we report a hitherto uncharacterised role for the ubiquitin-protein ligase NEDD4 in this process. XY Nedd4-deficient mice exhibit complete male-to-female gonadal sex reversal shown by the ectopic upregulation of Foxl2 expression at the time of gonadal sex determination as well as insufficient upregulation of Sox9. This sex reversal extends to germ cells with ectopic expression of SYCP3 in XY Nedd4-/- germ cells and significantly higher Sycp3 transcripts in XY and XX Nedd4-deficient mice when compared to both XY and XX controls. Further, Nedd4-/- mice exhibit reduced gonadal precursor cell formation and gonadal size as a result of reduced proliferation within the developing gonad as well as reduced Nr5a1 expression. Together, these results establish an essential role for NEDD4 in XY gonadal sex determination and development and suggest a potential role for NEDD4 in orchestrating these cell fate decisions through the suppression of the female pathway to ensure proper testis differentiation. |
Keywords: | Ovary Testis Gonads Animals Mice, Knockout Mammals Mice Cell Differentiation Gene Expression Regulation, Developmental Female Male SOX9 Transcription Factor 46, XX Testicular Disorders of Sex Development Nedd4 Ubiquitin Protein Ligases |
Rights: | © Crown 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
DOI: | 10.1038/s41419-022-04519-z |
Grant ID: | http://purl.org/au-research/grants/arc/DP150101448 http://purl.org/au-research/grants/nhmrc/GNT1103006 http://purl.org/au-research/grants/nhmrc/GNT2007739 |
Published version: | http://dx.doi.org/10.1038/s41419-022-04519-z |
Appears in Collections: | Medicine publications |
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File | Description | Size | Format | |
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hdl_134415.pdf | Published Version | 16.01 MB | Adobe PDF | View/Open |
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