Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/132410
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Type: Journal article
Title: No observed effect on brain vasculature of Alzheimer's disease-related mutations in the zebrafish presenilin 1 gene
Author: Barthelson, K.
Newman, M.
Nowell, C.J.
Lardelli, M.
Citation: Molecular Brain, 2021; 14(1):22-1-22-4
Publisher: BioMed Central; Springer Nature
Issue Date: 2021
ISSN: 1756-6606
1756-6606
Statement of
Responsibility: 
Karissa Barthelson, Morgan Newman, Cameron J. Nowell and Michael Lardelli
Abstract: Previously, we found that brains of adult zebrafish heterozygous for Alzheimer's disease-related mutations in their presenilin 1 gene (psen1, orthologous to human PSEN1) show greater basal expression levels of hypoxia responsive genes relative to their wild type siblings under normoxia, suggesting hypoxic stress. In this study, we investigated whether this might be due to changes in brain vasculature. We generated and compared 3D reconstructions of GFP-labelled blood vessels of the zebrafish forebrain from heterozygous psen1 mutant zebrafish and their wild type siblings. We observed no statistically significant differences in vessel density, surface area, overall mean diameter, overall straightness, or total vessel length normalised to the volume of the telencephalon. Our findings do not support that changes in vascular morphology are responsible for the increased basal expression of hypoxia responsive genes in psen1 heterozygous mutant brains.
Keywords: Zebrafish; vasculature; confocal laser scanning microscopy; 3D reconstruction
Rights: © The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
DOI: 10.1186/s13041-021-00734-5
Grant ID: http://purl.org/au-research/grants/nhmrc/GNT1126422
http://purl.org/au-research/grants/nhmrc/GNT1061006
Appears in Collections:Medicine publications

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