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https://hdl.handle.net/2440/127608
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Type: | Journal article |
Title: | Phosphorylation influences water and ion channel function of AtPIP2;1 |
Author: | Qiu, J. McGaughey, S.A. Groszmann, M. Tyerman, S.D. Byrt, C.S. |
Citation: | Plant, Cell and Environment, 2020; 43(10):2428-2442 |
Publisher: | Wiley |
Issue Date: | 2020 |
ISSN: | 0140-7791 1365-3040 |
Statement of Responsibility: | Jiaen Qiu, Samantha A. McGaughey, Michael Groszmann, Stephen D. Tyerman, Caitlin S. Byrt |
Abstract: | The phosphorylation state of two serine residues within the C-terminal domain of AtPIP2;1 (S280, S283) regulate its plasma membrane localisation in response to salt and osmotic stress. Here we investigated whether the phosphorylation state of S280 and S283 also influence AtPIP2;1 facilitated water and cation transport. A series of single and double S280 and S283 phosphomimic and phosphonull AtPIP2;1 mutants were tested in heterologous systems. In Xenopus laevis oocytes, phosphomimic mutants AtPIP2;1 S280D, S283D, and S280D/S283D had significantly greater ion conductance for Na+ and K+ , whereas the S280A single phosphonull mutant had greater water permeability. We observed a phosphorylation-dependent inverse relationship between AtPIP2;1 water and ion transport with a 10-fold change in both. The results revealed that phosphorylation of S280 and S283 influences the preferential facilitation of ion or water transport by AtPIP2;1. The results also hint that other regulatory sites play roles that are yet to be elucidated. Expression of the AtPIP2;1 phosphorylation mutants in Saccharomyces cerevisiae confirmed that phosphorylation influences plasma membrane localisation, and revealed higher Na+ accumulation for S280A and S283D mutants. Collectively, the results show that phosphorylation in the C-terminal domain of AtPIP2;1 influences its subcellular localisation and cation transport capacity. This article is protected by copyright. All rights reserved. |
Keywords: | Arabidopsis NSCC aquaporin gating osmotic stress potassium regulation salt stress sodium transport trafficking |
Description: | First published: 17 July 2020 |
Rights: | © 2020 John Wiley & Sons Ltd. |
DOI: | 10.1111/pce.13851 |
Grant ID: | http://purl.org/au-research/grants/arc/DP190102725 http://purl.org/au-research/grants/arc/FT180100476 http://purl.org/au-research/grants/arc/CE1401000015 |
Published version: | http://dx.doi.org/10.1111/pce.13851 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 4 |
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