Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/126830
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Type: Journal article
Title: 3D printing of cell-laden electroconductive bioink for tissue engineering application
Author: Tung, T.T.
Rastin, H.
Zhang, B.
Bi, J.
Hassan, K.
Losic, D.
Citation: Journal of Materials Chemistry B, 2020; 8(27):5862-5976
Publisher: Royal Society of Chemistry
Issue Date: 2020
ISSN: 2050-750X
2050-7518
Statement of
Responsibility: 
Hadi Rastin, Bingyang Zhang, Jingxiu Bi, Kamrul Hassan, Tran Thanh Tung and Dusan Losic
Abstract: Bioprinting is an emerging powerful fabrication method, which enables the rapid assembly of 3D bioconstructs with dispensing cell-laden bioinks in pre-designed locations. However, to translate this technology into real applications, there are still a number of challenges that need to be addressed. First, the current inks are generally composed of polymeric materials with poor electrical conductivity that mismatches with the native tissue environment. The second challenge associated with the 3D bioprinting of hydrogel-based bioinks is the fabrication of anatomical-size constructs without any loss of shape fidelity and resolution. To address these challenges, in this work, we introduced a biocompatible bioink associated with current 3D bioprinting by combining methylcellulose and kappa-carrageenan (MC/κCA) hydrogels with poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOT:PSS) conducting polymers. The prepared ink exhibited highly thixotropic behaviour, which could be tuned via changing the concentration of MC and κCA to obtain easy printing with high shape fidelity. The ink was able to fabricate physiological-scale constructs without requiring a secondary support bath. In addition, varying the concentration of PEDOT:PSS could control the electrical conductivity of the ink. Moreover, the encapsulated human embryonic kidney 293 (HEK-293) cells in bulk hydrogels and 3D bioprinted structures maintained high cell viability (>96%) over a week, confirming the in vitro biocompatibility of the ink. Overall, these findings indicate that the MC/κCA/PEDOT:PSS bioink can be promising in biomedical applications, which improved the electroconductivity of bioinks and can exploit the advantage of conductive polymers in the 3D bioprinting technology.
Keywords: Cells, Cultured
Humans
Polystyrenes
Polymers
Carrageenan
Methylcellulose
Biocompatible Materials
Hydrogels
Cross-Linking Reagents
Tissue Engineering
Electric Conductivity
Tissue Scaffolds
HEK293 Cells
Printing, Three-Dimensional
Bridged Bicyclo Compounds, Heterocyclic
Rights: This journal is©The Royal Society of Chemistry 2020.
DOI: 10.1039/d0tb00627k
Grant ID: http://purl.org/au-research/grants/arc/IH150100003)
Appears in Collections:ARC Research Hub for Graphene Enabled Industry Transformation publications
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