Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/124614
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Type: Journal article
Title: A small-scale extraction pipeline for rapid analysis of seed mucilage characteristics
Author: Cowley, J.M.
Herliana, L.
Neumann, K.
Silvano, C.
Cerne, V.
Burton, R.
Citation: Plant Methods, 2020; 16(10):1-12
Publisher: BioMed Central
Issue Date: 2020
ISSN: 1746-4811
1746-4811
Statement of
Responsibility: 
James M. Cowley, Lina Herliana, Kylie A. Neumann, Silvano Ciani, Virna Cerne and Rachel A. Burton
Abstract: Background: Myxospermy is a process by which the external surfaces of seeds of many plant species produce mucilage—a polysaccharide-rich gel with numerous fundamental research and industrial applications. Due to its functional properties the mucilage can be difficult to remove from the seed and established methods for mucilage extraction are often incomplete, time-consuming and unnecessarily wasteful of precious seed stocks. Results: Here we tested the efficacy of several established protocols for seed mucilage extraction and then downsized and adapted the most effective elements into a rapid, small-scale extraction and analysis pipeline. Within 4 h, three chemically- and functionally-distinct mucilage fractions were obtained from myxospermous seeds. These fractions were used to study natural variation and demonstrate structure–function links, to screen for known mucilage quality markers in a field trial, and to identify research and industry-relevant lines from a large mutant population. Conclusion: The use of this pipeline allows rapid analysis of mucilage characteristics from diverse myxospermous germplasm which can contribute to fundamental research into mucilage production and properties, quality testing for industrial manufacturing, and progressing breeding efforts in myxospermous crops.
Keywords: Mucilage; Myxospermy; Extraction; Polysaccharide; Plantago ovata; Flax; Chia; Psyllium
Rights: © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativeco mmons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/ zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
RMID: 1000016457
DOI: 10.1186/s13007-020-00569-6
Grant ID: http://purl.org/au-research/grants/arc/110001007
http://purl.org/au-research/grants/arc/140100008
Appears in Collections:Agriculture, Food and Wine publications

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