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https://hdl.handle.net/2440/119408
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Type: | Journal article |
Title: | Globular structures in roots accumulate phosphorus to extremely high concentrations following phosphorus addition |
Author: | Ryan, M.H. Kaur, P. Nazeri, N.K. Clode, P.L. Keeble-Gagnère, G. Doolette, A.L. Smernik, R.J. Van Aken, O. Nicol, D. Maruyama, H. Ezawa, T. Lambers, H. Millar, A.H. Appels, R. |
Citation: | Plant, Cell and Environment, 2019; 42(6):1987-2002 |
Publisher: | Wiley Online Library |
Issue Date: | 2019 |
ISSN: | 0140-7791 1365-3040 |
Statement of Responsibility: | Megan H. Ryan, Parwinder Kaur, Nazanin K. Nazeri, Peta L. Clode, Gabriel Keeble‐Gagnère |
Abstract: | Crops with improved uptake of fertilizer phosphorus (P) would reduce P losses and confer environmental benefits. We examined how P-sufficient 6-week-old soil-grown Trifolium subterraneum plants, and 2-week-old seedlings in solution culture, accumulated P in roots after inorganic P (Pi) addition. In contrast to our expectation that vacuoles would accumulate excess P, after 7 days, X-ray microanalysis showed that vacuolar [P] remained low (<12 mmol kg-1 ). However, in the plants after P addition, some cortex cells contained globular structures extraordinarily rich in P (often >3,000 mmol kg-1 ), potassium, magnesium, and sodium. Similar structures were evident in seedlings, both before and after P addition, with their [P] increasing threefold after P addition. Nuclear magnetic resonance (NMR) spectroscopy showed seedling roots accumulated Pi following P addition, and transmission electron microscopy (TEM) revealed large plastids. For seedlings, we demonstrated that roots differentially expressed genes after P addition using RNAseq mapped to the T. subterraneum reference genome assembly and transcriptome profiles. Among the most up-regulated genes after 4 hr was TSub_g9430.t1, which is similar to plastid envelope Pi transporters (PHT4;1, PHT4;4): expression of vacuolar Pi-transporter homologs did not change. We suggest that subcellular P accumulation in globular structures, which may include plastids, aids cytosolic Pi homeostasis under high-P availability. |
Keywords: | X-ray microanalysis nuclear magnetic resonance spectroscopy phosphorous efficient crops plastids roots vacuoles |
Rights: | © 2019 John Wiley & Sons Ltd. |
DOI: | 10.1111/pce.13531 |
Grant ID: | http://purl.org/au-research/grants/arc/FT140100103 http://purl.org/au-research/grants/arc/CE140100008 |
Published version: | http://dx.doi.org/10.1111/pce.13531 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 8 |
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