Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/115402
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Type: | Journal article |
Title: | miR-542-3p prevents ovariectomy-induced osteoporosis in rats via targeting SFRP1 |
Author: | Zhang, X. Zhu, Y. Zhang, C. Liu, J. Sun, T. Li, D. Na, Q. Xian, C.J. Wang, L. Teng, Z. |
Citation: | Journal of Cellular Physiology, 2018; 233(9):6798-6806 |
Publisher: | Wiley |
Issue Date: | 2018 |
ISSN: | 0021-9541 1097-4652 |
Statement of Responsibility: | Xiguang Zhang, Yun Zhu, Chuanlin Zhang, Jianping Liu, Tianming Sun, Dan Li, Qiang Na, Cory J. Xian, Liping Wang, Zhaowei Teng |
Abstract: | Secreted frizzled-related protein-1 (SFRP1) is a negative regulatory molecule of the WNT signaling pathway and serves as a therapeutic target for bone formation in osteoporosis. In this study, we first established an ovariectomized (OVX) rat model to simulate postmenopausal osteoporosis and found significant changes in miR-542-3p and sFRP1 expression by RNA sequencing and qRT-PCR. In addition, there was a significant negative correlation between miR-542-3p and sFRP1 mRNA levels in postmenopausal women with osteoporosis. We found that miR-542-3p inhibited the expression of sFRP1 mRNA by luciferase reporter assay. When the miR-542-3p binding site in sFRP1 3'UTR was deleted, it did not affect its expression. Western blot results showed that miR-542-3p inhibited the expression of SFRP1 protein. The expression of SFRP1 was significantly increased in osteoblast-induced mesenchymal stem cells (MSC), whereas the expression of miR-542-3p was significantly decreased. And miR-542-3p transfected MSCs showed a significant increase in osteoblast-specific marker expression, indicating that miR-542-3p is necessary for MSC differentiation. Inhibition of miR-542-3p reduced bone formation, confirmed miR-542-3p play a role in bone formation in vivo. In general, these data suggest that miR-542-3p play an important role in bone formation via inhibiting SFRP1 expression and inducing osteoblast differentiation. |
Keywords: | differentiation miRNA osteoporosis SFRP1 |
Rights: | © 2018 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
DOI: | 10.1002/jcp.26430 |
Grant ID: | 81660156 81760136 81671928 2014FZ048 2017FE468 (-181), 2018 http://purl.org/au-research/grants/nhmrc/1094606 http://purl.org/au-research/grants/nhmrc/1042105 |
Published version: | http://dx.doi.org/10.1002/jcp.26430 |
Appears in Collections: | Aurora harvest 8 Paediatrics publications |
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hdl_115402.pdf | Published version | 2.21 MB | Adobe PDF | View/Open |
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