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https://hdl.handle.net/2440/114948
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dc.contributor.author | Harris, R. | - |
dc.contributor.author | Marmion, B.P. | - |
dc.contributor.author | Varkanis, G. | - |
dc.contributor.author | Kok, T. | - |
dc.contributor.author | Lunn, B. | - |
dc.contributor.author | Martin, J. | - |
dc.date.issued | 1988 | - |
dc.identifier.citation | Epidemiology and Infection, 1988; 101(3):685-694 | - |
dc.identifier.issn | 0950-2688 | - |
dc.identifier.issn | 1469-4409 | - |
dc.identifier.uri | http://hdl.handle.net/2440/114948 | - |
dc.description.abstract | The efficiency of the direct detection of Mycoplasma pneumoniae in respiratory exudates by an antigen capture, indirect enzyme immunoassay (Ag-EIA), has been compared with its detection with a cDNA probe ('Gen-Probe assay') directed against the specific ribosomal RNA sequences of the organism ('Mycoplasma pneumoniae Rapid Diagnostic System', Gen-Probe, San Diego, California). Both assays showed excellent specificity against a range of mycoplasma species suspended in negative nasopharyngeal aspirates; only M. pneumoniae and M. genitalium reacted. In experiments with graded doses of viable M. pneumoniae cells suspended in negative nasopharyngeal aspirate, the Gen-Probe assay was more sensitive than Ag-EIA; detection limits were respectively 2 X 10(3) c.f.u./ml (3.2 X 10(5) genomes) and 2.5 X 10(4) c.f.u./ml (4 X 10(6) genomes); detection levels 10-100 times less sensitive than culture. The two assays were also tested on nasopharyngeal aspirates or sputum specimens from 90 patients with respiratory infection; 67 of these were culture- or seronegative for M. pneumoniae and 23 were culture- or seropositive. Ag-EIA detected 21 (91%) of the latter but the Gen-Probe assay detected only 5 (22%). Both assays were negative with the 67 culture-/sero-negatives; there were no Gen-Probe assay positive/Ag-EIA negatives. Overall, it is concluded that although Ag-EIA and the Gen-Probe assay are effective substitutes for culture as a diagnostic procedure, there is a significant problem with samples which are culture-negative and from patients who have good serological evidence of current infection. Possible reasons for the disparity between the two assays are advanced. | - |
dc.description.statementofresponsibility | R. Harris, B. P. Marmion, G. Varkanis, T. Kok, B. Lunn, and J. Martin | - |
dc.language.iso | en | - |
dc.publisher | Cambridge University Press | - |
dc.rights | © Cambridge University Press 1988 | - |
dc.source.uri | http://dx.doi.org/10.1017/s0950268800029563 | - |
dc.subject | Mycoplasma pneumoniae | - |
dc.subject | DNA Probes | - |
dc.subject | RNA, Ribosomal | - |
dc.title | Laboratory diagnosis of Mycoplasma pneumoniae infection: 2. Comparison of methods for the direct detection of specific antigen or nucleic acid sequences in respiratory exudates | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1017/S0950268800029563 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 8 Medicine publications |
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