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https://hdl.handle.net/2440/114947
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Type: | Journal article |
Title: | Laboratory diagnosis of Mycoplasma pneumoniae infection: 1. Direct detection of antigen in respiratory exudates by enzyme immunoassay |
Author: | Kok, T.W. Varkanis, G. Marmion, B.P. Martin, J. Esterman, A. |
Citation: | Epidemiology and Infection, 1988; 101(3):669-684 |
Publisher: | Cambridge University Press |
Issue Date: | 1988 |
ISSN: | 0950-2688 1469-4409 |
Statement of Responsibility: | T.-W. Kok, G. Varkanis, B. P. Marmion, J. Martin and A. Esterman |
Abstract: | Direct and indirect antigen capture enzyme immunoassays (Ag-EIA) have been developed for the detection of Mycoplasma pneumoniae in nasopharyngeal aspirates or sputum from respiratory infection. The sensitivity of the two Ag-EIA were similar, but the indirect method using polyclonal rabbit and guinea-pig antisera was more convenient. The Ag-EIA had a detection limit of 10(4-4.5) colony-forming units/ml of sample. It was specific for M. pneumoniae and gave a low level response with M. genitalium. There were no cross-reactions with 10 other species of mycoplasmas. Tests with a wide range of bacteria and chlamydia group antigen, representing agents sometimes found in the respiratory tract, were also negative. At the current level of development, the Ag-EIA detected about 90% of specimens that were also positive for culture; 43% of specimens from culture-negative--seropositive patients gave a positive result. The overall pattern of results indicated that while antigen detection is a quick and effective substitute for the slow culture method, serological examination for specific IgM antibody is also necessary to give a complete diagnostic coverage. |
Keywords: | Nasopharynx Mycoplasma pneumoniae Antigens, Bacterial |
Rights: | © Cambridge University Press 1988 |
DOI: | 10.1017/S0950268800029551 |
Published version: | http://dx.doi.org/10.1017/s0950268800029551 |
Appears in Collections: | Aurora harvest 8 Medicine publications |
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