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Type: Journal article
Title: Functional analysis of overexpressed PtDRS1 involved in abiotic stresses enhances growth in transgenic poplar
Author: Mohammadi, K.
Movahedi, A.
Maleki, S.
Sun, W.
Zhang, J.
Almasi Zadeh Yaghuti, A.
Nourmohammadi, S.
Zhuge, Q.
Citation: Plant Physiology and Biochemistry, 2018; 126:22-31
Publisher: Elsevier
Issue Date: 2018
ISSN: 0981-9428
Statement of
Kourosh Mohammadi, Ali Movahedi, Samaneh sadat Maleki, Weibo Sun, Jiaxin Zhang, Amir Almasi Zadeh Yaghutia, Saeed Nourmohammadi, Qiang Zhuge
Abstract: Drought and salinity are two main abiotic stressors that can disrupt plant growth and survival. Various biotechnological approaches have been used to alleviate the problem of drought stress by improving water stress resistance in forestry and agriculture. The drought sensitive 1 (DRS1) gene acts as a regulator of drought stress, identified in human, yeast and some model plants, such as Arabidopsis thaliana, but there have been no reports of DRS1 transformation in poplar plants to date. In this study, we transformed the DRS1 gene from Populus trichocarpa into Populus deltoides × Populus euramericana 'Nanlin895' using Agrobacterium tumefaciens-mediated transformation. We confirmed that the DRS1 gene was transformed into 'Nanlin895' poplar genomes using reverse transcription polymerase chain reaction (PCR), multiplex PCR, real-time PCR, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. All transformed and wild-type (WT) plants were then transferred into a greenhouse for complementary experiments. We analyzed the physiological and biochemical responses of transgenic plants under drought and salt stresses in the greenhouse, and the results were compared with control WT plants. Responses to abiotic stress were greater in transgenic plants compared with WT. Based on our results, introduction of the DRS1 gene into poplar 'Nanlin895' plants significantly enhanced the resistance of those plants to water deficit and high salinity, allowing higher growth rates of roots and shoots in those plants. Additionally, the clawed root rate increased in transformed poplars grown in culture media or in soil, and improved survival under drought and salt stress conditions.
Keywords: Abiotic stresses
Physiological and biochemical responses
Transgenic poplar
Rights: © 2018 Elsevier Masson SAS. All rights reserved.
DOI: 10.1016/j.plaphy.2018.01.023
Appears in Collections:Aurora harvest 8
Environment Institute publications

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