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https://hdl.handle.net/2440/11426
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Type: | Journal article |
Title: | Molecular characterisation of mutants of the acetate regulatory gene facB of Aspergillus nidulans |
Author: | Todd, R. Kelly, J. Davis, M. Hynes, M. |
Citation: | Fungal Genetics and Biology, 1997; 22(2):92-102 |
Publisher: | Elsevier BV |
Issue Date: | 1997 |
ISSN: | 1087-1845 1096-0937 |
Abstract: | The facB gene of Aspergillus nidulans encodes a DNA binding transcriptional activator required for growth on acetate as a sole carbon source. FacB contains N-terminal GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA binding and leucine zipper-like heptad repeat motifs and central and C-terminal acidic alpha-helical regions. facB recessive loss of function mutants are deficient in acetate induction of acetyl-CoA synthase, isocitrate lyase, malate synthase, acetamidase, and NADP-isocitrate dehydrogenase. Characterization of lesions in facB mutant alleles has localized important functional regions of the FacB protein. Two extreme mutants are shown to lack the C-terminal region of the protein. Two temperature sensitive mutants contain amino acid substitutions in the DNA binding domain and are shown to affect acetate induction of amdS-lacZ expression and confer temperature sensitive in vitro DNA binding. Two temperature sensitive facB mutations result in thermolability of acetyl-CoA synthase, isocitrate lyase, and malate synthase but not acetamidase or NADP-isocitrate dehydrogenase in crude extracts. This suggests that FacB may have a structural role in acetate metabolism in addition to its regulatory function. |
Keywords: | Escherichia coli Aspergillus nidulans Acetates Amidohydrolases Trans-Activators Fungal Proteins Recombinant Fusion Proteins Sequence Analysis, DNA Enzyme Stability Temperature Enzyme Induction Sequence Deletion Amino Acid Sequence Zinc Fingers Mutation Alleles Genes, Fungal Molecular Sequence Data |
DOI: | 10.1006/fgbi.1997.1007 |
Published version: | http://dx.doi.org/10.1006/fgbi.1997.1007 |
Appears in Collections: | Aurora harvest 2 Genetics publications |
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