Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/112728
Type: | Theses |
Title: | Analysis of genetic and epigenetic variability of grapevine cultivar Shiraz in Barossa Valley |
Author: | Xie, Huahan |
Issue Date: | 2016 |
School/Discipline: | School of Agriculture, Food and Wine |
Abstract: | The wine industry in Australia has rapidly increased in past decades and wine quality is the key factor to settle an appropriate price for wine. The wine quality mostly relies on the quality of grape in different environmental condition, whereas this specific environmental conditions are described as Terroir. In this study, we applied both Methylation Sensitive Amplification Polymorphism (MSAP) and methylation sensitive Genotyping by Sequencing to investigating the variation of DNA methylation between plants of Shiraz variety collected from 22 vineyards of six regions in the Barossa Valley. Results shows that different DNA methylation patterns of regions were obtained due to their various environmental conditions. However, two vineyards show a significant difference compared to other vineyards which may due to accumulated somatic mutation. To reduce genetic effect, we selected samples which were the same clones with the same pruning method. The result suggests that different farm managements can induce variability in DNA methylation. Since both environmental conditions and farming systems define a terroir, we infer that DNA methylation may play an important role in this concept. |
Advisor: | Rodriguez Lopez, Carlos Marcelino Konate, Moumouni |
Dissertation Note: | Thesis (M.Bio.(PB)) -- University of Adelaide, Masters of Biotechnology (Plant Biotechnology), School of Agriculture, Food and Wine, 2016. |
Keywords: | coursework DNA methylation grapevine wine terroir MSAP ms-GBS epigenetic |
Provenance: | Masters of Biotechnology (Plant Biotechnology) by coursework |
Appears in Collections: | School of Agriculture, Food and Wine |
Files in This Item:
File | Description | Size | Format | |
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09jmx6.pdf | Title page, abstract and table of contents only | 53.96 kB | Adobe PDF | View/Open |
Restricted Restricted Access | Archival - Library staff access only | 2.01 MB | Adobe PDF | View/Open |
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