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Type: Theses
Title: Intergenomic SNPs reveal putative spontaneous chromosomal interchanges between chromosomes 7A and 7D of wheat
Author: Nguyen, Phuoc Xuan
Issue Date: 2016
School/Discipline: School of Agriculture, Food and Wine
Abstract: Langdon 7D(7A) and 7D(7B) durum substitution lines were crossed with DBA-Aurora durum wheat to introgress a lutein esterification gene,TaGelp1, from chromosome 7D onto its homoeologues 7A and 7B. Genotyping-by-sequencing based on DNA samples from durum wheat and bread wheat revealed single nucleotide polymorphism (SNPs) among the group-7 chromosomes. Sixteen KASP markers were developed and to be able to differentiate among these chromosomes. Nine 7A-7D markers were used to characterise progeny populations to search for dissociation of molecular markers which may indicate chromosomal recombination. Evidence of possible 7A-7D recombination was found in a small number of progeny (less than 4%). Most of the putative marker dissociations were in the centromeric region but one plant was found to carry only a small distal fragment of 7DS including TaGelp1. The findings suggest crossing normal durum with Langdon 7D(7A) combined with KASP marker assistance can be applied as a method to introgress and assess genes from chromosome 7D onto its homoeologues without resorting to use of wheat with the Ph1 deletion.
Advisor: Mather, Diane Elizabeth
Dissertation Note: Thesis (M.Bio.(PB)) -- University of Adelaide, Masters of Biotechnology (Plant Biotechnology), School of Agriculture, Food and Wine, 2016.
Keywords: coursework
Triticum turgidum subsp. durum
lutein esterification
KASP marker
homoeologous recombination
Description: Title page, abstract and table of contents only. The complete thesis in print form is available from the University of Adelaide Library.
Provenance: Masters of Biotechnology (Plant Biotechnology) by coursework
Appears in Collections:School of Agriculture, Food and Wine

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