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Type: Theses
Title: Transcriptome comparison of Shiraz (Vitis vinifera) grapevines in distinct sub-regions of the Barossa Valley
Author: Sai, Na
Issue Date: 2016
School/Discipline: School of Agriculture, Food and Wine
Abstract: Studying the interaction between grapevines and the environment may provide insights of how terroir drives unique characters in wine. Analysing changes in gene expression between different environmental conditions provides a first step in understanding genes that may play a role in grapevine adaption. We, therefore, carried out RNA-seq analysis on Shiraz grapevine leaf tissue harvested from two sub-regions of the Barossa Valley to investigate whether gene expression changes occurred in response to two important environmental factors for plant growth, temperature and elevation. Young leaves from three vineyards in the Barossa central ground and three in the Eden Valley were sampled at budburst. The transcriptome profiling of all samples was clustered by vineyard and separated by region. In total, 429 genes showed significant changes in gene expression between two regions (FDR < 0.001). Among the differentially expressed genes, we found a subset of genes enriched in Gene Ontology (GO) terms that are related to environmental response, including abiotic stress and external biotic stress (Q-value < 0.05). Our study provides preliminary analysis of transcriptome changes in different sub-regions of Barossa Valley and identified potential candidate genes involved in adaptive responses under different environmental condition.
Advisor: Gilliham, Matthew
Rodriguez Lopez, Carlos Marcelino
Dissertation Note: Thesis (M.Bio.(PB)) -- University of Adelaide, Masters of Biotechnology (Plant Biotechnology), School of Agriculture, Food and Wine, 2016
Keywords: coursework
transcriptome analysis
grapevine adaption
Barossa Valley
Description: Title page, abstract and table of contents only. The complete thesis in print form is available from the University of Adelaide Library.
Provenance: Masters of Biotechnology (Plant Biotechnology) by coursework
Appears in Collections:School of Agriculture, Food and Wine

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