Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/112020
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Association between Extracellular Material and Biofilm Formation in Response to Sodium Hypochlorite by Clinical Isolates of Enterococcus faecalis
Author: Yoo, A.
Rossi-Fedele, G.
Kidd, S.
Rogers, A.
Zilm, P.
Citation: Journal of Endodontics, 2018; 44(2):269-273
Publisher: American Association of Endodontists
Issue Date: 2018
ISSN: 0099-2399
1878-3554
Statement of
Responsibility: 
Austin Yoo, Giampiero Rossi-Fedele, Stephen P. Kidd, Anthony H.Rogers, Peter S. Zilm
Abstract: Extracellular material (ECM) surrounding Enterococcus faecalis may play a role in increasing resistance to environmental stresses. Our aim was to determine ECM levels in response to subminimal inhibitory concentrations of sodium hypochlorite (sub-MIC/NaOCl) or anaerobic growth and determine the impact on biofilm development.From 37 E. faecalis clinical strains, 19 were selected according to their biofilm-producing ability by using a crystal violet biofilm assay: 10 strong, 4 intermediate, and 5 non-biofilm producers. Biofilm assays were subsequently performed on all strains when subjected to sub-MIC/NaOCl. All strains were evaluated for ECM production under aerobic and anaerobic conditions and with sub-MIC/NaOCl. ECM production was assessed by using scanning electron microscopy. Double-blinded independent assessors were used to score levels of ECM production. The esp gene was detected by using polymerase chain reaction. Gelatinase activity was determined by using Todd-Hewitt and gelatin agar.In aerobic conditions, ECM was expressed in all strains. In the presence of sub-MIC/NaOCl, of the 10 strong biofilm producers, 5 increased their ECM production, and 4 showed increased biofilm growth. Two strains had less ECM production and showed decreased biofilm growth. One isolate demonstrated no observable changes. Most non-biofilm producers demonstrated no observable differences in ECM production, although 1 strain increased biofilm growth. ECM production in anaerobic conditions was highly variable. The esp gene (n = 15) and gelatinase activity (n = 7) were evident among the isolates.Clonal diversity among strains of E. faecalis suggests that some strong biofilm producers can upregulate ECM production and increase biofilm growth in response to sub-MIC/NaOCl.
Keywords: Biofilm
Enterococcus faecalis
clonal diversity
extracellular material
stress response
Rights: © 2017 American Association of Endodontists.
DOI: 10.1016/j.joen.2017.08.025
Published version: http://dx.doi.org/10.1016/j.joen.2017.08.025
Appears in Collections:Aurora harvest 3
Dentistry publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.