Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/111544
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Type: | Journal article |
Title: | Lesion development is modulated by the natural estrous cycle and mouse strain in a minimally invasive model of endometriosis |
Author: | Dodds, K.N. Beckett, E.A. Evans, S.F. Hutchinson, M.R. |
Citation: | Biology of Reproduction, 2017; 97(6):810-821 |
Publisher: | Society for the Study of Reproduction |
Issue Date: | 2017 |
ISSN: | 0006-3363 1529-7268 |
Statement of Responsibility: | Kelsi N. Dodds, Elizabeth A. H. Beckett, Susan F. Evans and Mark R. Hutchinson |
Abstract: | Many rodent models of endometriosis are invasive, involving surgery to implant donor endometrial tissue into recipient animals. Moreover, few studies have compared and contrasted lesions between rodent strains and estrous stages without exogenous hormone manipulation. This is despite extensive data demonstrating that genetic and hormonal factors can influence endometriosis progression. Here, we have refined a minimally-invasive model of endometriosis using naturally cycling mice (donor and recipient matched for cycle phase) to investigate lesion development in two different strains (C57BL/6 and Balb/C), induced in estrous stages of high and low estrogen (proestrus or estrus, respectively), and with varying amounts of donor endometrial tissue (7.5-40 mg), injected intraperitoneally. The overall probability of developing endometriosis-like lesions was higher in proestrus than estrus, and increased with greater masses of donor tissue. Similarly, the total number of lesions (0-3) increased from 7.5 to 40 mg, and was significantly greater in proestrus C57BL/6 mice but not Balb/Cs. The dominant lesion type also differed between mouse strains; C57BL/6 mice were more likely to develop dense-type lesions, whereas Balb/C mice developed a greater proportion of cystic-type. This data further supports a role for estrogen in the development of endometriosis, and that genetic variance can influence the degree and characteristics of lesions. Our minimally-invasive model would be beneficial for studies with outcome measurements particularly sensitive to incisional injury, such as pain, or alterations to sex hormones, including fertility. |
Keywords: | endometriosis endometrium mouse model mouse strain estrogen estrous cycle |
Description: | Advance Access Publication Date: 24 October 2017 |
Rights: | © The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved |
DOI: | 10.1093/biolre/iox132 |
Grant ID: | http://purl.org/au-research/grants/arc/DP110100297 |
Published version: | http://dx.doi.org/10.1093/biolre/iox132 |
Appears in Collections: | Aurora harvest 8 Physiology publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_111544.pdf | Accepted version | 488.17 kB | Adobe PDF | View/Open |
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