Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/109121
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Vacuolation activity and intracellular trafficking of ArtB, the binding subunit of an AB5 toxin produced by Salmonella enterica Serovar Typhi
Author: Herdman, B.P.
Paton, J.C.
Wang, H.
Beddoe, T.
Paton, A.W.
Citation: Infection and Immunity, 2017; 85(8):e00214-17-1-e00214-17-8
Publisher: American Society for Microbiology
Issue Date: 2017
ISSN: 0019-9567
1098-5522
Editor: McCormick, B.
Statement of
Responsibility: 
Brock P. Herdman, James C. Paton, Hui Wang, Travis Beddoe, Adrienne W. Paton
Abstract: Various Salmonella enterica serovars, including S. enterica serovar Typhi, encode an AB5 toxin (ArtAB), the A subunit of which is an ADP-ribosyltransferase related to the S1 subunit of pertussis toxin. However, although the A subunit is able to catalyze ADP-ribosylation of host G proteins, a cytotoxic phenotype has yet to be identified for the holotoxin. Here we show that its B subunit pentamer (ArtB) binds to receptors on the surface of Vero (African green monkey kidney) cell, CHO (Chinese hamster ovary) cell, U937 (human monocyte) cell, and HBMEC (human brain microvascular endothelial cell) lines. Moreover, ArtB induced marked vacuolation in all cell lines after 4 h of incubation. Further studies in Vero cells showed that vacuolation was inhibited by bafilomycin A1 and was dependent on the clathrin-mediated uptake of ArtB. Vacuolation was also inhibited by treatment of cells with neuraminidase, indicating that sialylated glycans are functional receptors for ArtB. Confocal colocalization studies indicated that after cell binding and internalization, ArtB undergoes retrograde transport via early endosomes, the trans-Golgi network, and the Golgi apparatus, reaching the endoplasmic reticulum (ER) after approximately 2 h. The onset of vacuolation also coincided with gross cytoskeletal reorganization. At later time points, ArtB colocalized with ERTracker Red in the vacuolar membrane, implying that vacuolation is a consequence of ER disorganization. Thus, the isolated B subunit of this cryptic AB5 toxin has significant effects on target cells with the potential to contribute directly to pathogenesis independently of the catalytic A subunit.
Keywords: AB5 toxins; B subunit; Salmonella; vacuolation
Rights: Copyright © 2017 American Society for Microbiology. All Rights Reserved.
DOI: 10.1128/iai.00214-17
Grant ID: NHMRC
Published version: http://dx.doi.org/10.1128/iai.00214-17
Appears in Collections:Aurora harvest 3
Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.