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dc.contributor.advisorBurton, Rachel Anita-
dc.contributor.advisorCollins, Helen-
dc.contributor.advisorByrt, Caitlin S.-
dc.contributor.advisorFincher, Geoffrey Bruce-
dc.contributor.authorErmawar, Riksfardini Annisa-
dc.description.abstractThe amount of (1,3;1,4)-β-D-glucans or mixed-linkage-β-glucans (MLG) in biofuel crops is of interest because this is a source of fermentable hexose sugar. Recent modification of (1,3;1,4)-β-glucan in barley has opened the possibility of increasing the (1,3;1,4)-β-glucan amount in other plants, including bioethanol crops such as sorghum, a C₄ grass. As S. viridis is a model plant for studying C₄ biofuel crops the characteristics of MLG accumulation in S. viridis is of interest. The initial study determines the levels, structure and distribution of MLG in various vegetative tissues and grain in S. viridis, and investigates the relationship with the transcript levels of Cellulose synthase-like (Csl) genes, i.e. genes involve in MLG synthesis. Modification of MLG amount was tested by generating transformants of S. viridis with over-expressing Csl F6 driven by either the oat globulin promoter (ProASGLO) or the constitutive 35S promoter (Pro35S) from barley (HvCslF6) and sorghum (SbCslF6). In addition, a previous study on the over-expression of the (1,3;1,4)-β-glucan synthase in transgenic barley showed an excessive deposition of the polysaccharide in many cell types causing vascular suffocation and sometimes plant death. To resolve this problem this study also aim to identify a sorghum promoter which may be used to regulate the over-expression of (1,3;1,4)-β-D-glucan synthase genes (such as CslF and CslH) and restrict it to certain specific cell types only. Five putative sorghum mesophyll cell-specific promoters from genes involved in C₄ photosynthesis have been fused with a β-glucuronidase (uidA) cDNA. All modifications were tested in planta using stable Agrobacterium-mediated transformation.en
dc.subjectCellulose synthase geneen
dc.subjectAgrobacterium-mediated transformationen
dc.titleMetabolic engineering of C₄ grasses for biofuel applicationsen
dc.contributor.schoolSchool of Agriculture, Food and Wineen
dc.provenanceThis electronic version is made publicly available by the University of Adelaide in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exceptions. If you are the owner of any included third party copyright material you wish to be removed from this electronic version, please complete the take down form located at:
dc.description.dissertationThesis (Ph.D.) (Research by Publication) -- University of Adelaide, School of Agriculture, Food and Wine, 2016.en
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