Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/103093
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Type: | Journal article |
Title: | A comparative gene analysis with rice identified orthologous group II HKT genes and their association with Na⁺ concentration in bread wheat |
Other Titles: | A comparative gene analysis with rice identified orthologous group II HKT genes and their association with Na(+) concentration in bread wheat |
Author: | Ariyarathna, H.A.C.K. Oldach, K.H. Francki, M.G. |
Citation: | BMC Plant Biology, 2016; 16(1):21-1-21-20 |
Publisher: | BioMed Central |
Issue Date: | 2016 |
ISSN: | 1471-2229 1471-2229 |
Statement of Responsibility: | H. A. Chandima K. Ariyarathna, Klaus H. Oldach and Michael G. Francki |
Abstract: | Background: Although the HKT transporter genes ascertain some of the key determinants of crop salt tolerance mechanisms, the diversity and functional role of group II HKT genes are not clearly understood in bread wheat. The advanced knowledge on rice HKT and whole genome sequence was, therefore, used in comparative gene analysis to identify orthologous wheat group II HKT genes and their role in trait variation under different saline environments. Results: The four group II HKTs in rice identified two orthologous gene families from bread wheat, including the known TaHKT2;1 gene family and a new distinctly different gene family designated as TaHKT2;2. A single copy of TaHKT2;2 was found on each homeologous chromosome arm 7AL, 7BL and 7DL and each gene was expressed in leaf blade, sheath and root tissues under non-stressed and at 200 mM salt stressed conditions. The proteins encoded by genes of the TaHKT2;2 family revealed more than 93% amino acid sequence identity but ≤52% amino acid identity compared to the proteins encoded by TaHKT2;1 family. Specifically, variations in known critical domains predicted functional differences between the two protein families. Similar to orthologous rice genes on chromosome 6L, TaHKT2;1 and TaHKT2;2 genes were located approximately 3 kb apart on wheat chromosomes 7AL, 7BL and 7DL, forming a static syntenic block in the two species. The chromosomal region on 7AL containing TaHKT2;1 7AL-1 co-located with QTL for shoot Na(+) concentration and yield in some saline environments. Conclusion: The differences in copy number, genes sequences and encoded proteins between TaHKT2;2 homeologous genes and other group II HKT gene families within and across species likely reflect functional diversity for ion selectivity and transport in plants. Evidence indicated that neither TaHKT2;2 nor TaHKT2;1 were associated with primary root Na(+) uptake but TaHKT2;1 may be associated with trait variation for Na(+) exclusion and yield in some but not all saline environments. |
Keywords: | Group II HKT; IWGS; Rice genome; Na+ exclusion |
Rights: | © 2016 Ariyarathna et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
DOI: | 10.1186/s12870-016-0714-7 |
Published version: | http://dx.doi.org/10.1186/s12870-016-0714-7 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 3 |
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hdl_103093.pdf | Published Version | 1.78 MB | Adobe PDF | View/Open |
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